Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from Ganoderma leucocontextum fruiting bodies in vitro and in vivo

被引：14

作者：

Gao, Xiong ^{[1
]}

Zeng, Ranhua ^{[2
]}

Qi, Jiayi ^{[2
]}

Ho, Chi-Tang ^{[3
]}

Li, Bin ^{[2
,4
]}

Chen, Zhongzheng ^{[2
,4
]}

Chen, Shaodan ^{[1
]}

Xiao, Chun ^{[1
]}

Hu, Huiping ^{[1
]}

Cai, Manjun ^{[1
]}

Xie, Yizhen ^{[1
,5
,6
]}

Wu, Qingping ^{[1
]}

机构：

[1] Guangdong Acad Sci, Inst Microbiol,Key Lab Agr Microbi & Precis Appli, State Key Lab Appl Microbiol Southern China,Minis, Guangdong Prov Key Lab Microbial Safety & Hlth, Guangzhou 510070, Peoples R China

[2] South China Agr Univ, Coll Food Sci, 483 Wushan St, Guangzhou 510642, Peoples R China

[3] Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA

[4] South China Agr Univ, Guangdong Prov Key Lab Nutraceut & Funct Foods, Guangzhou 510642, Peoples R China

[5] Guangdong Yuewei Edible Fungi Technol Co Ltd, Guangzhou 510663, Peoples R China

[6] Guangdong Yuewei Biotechnol Co Ltd, Zhaoqing 526000, Peoples R China

来源：

FOOD CHEMISTRY-X | 2022年 / 14卷

关键词：

Ganoderma leucocontextum; Heteropolysaccharide; Immunostimulatory activity; Molecular mechanism; Immunosuppressed mice; B SIGNALING PATHWAYS; IMMUNOSTIMULATORY ACTIVITY; MACROPHAGE ACTIVATION; POLYSACCHARIDE; ANTIOXIDANT; DECTIN-1;

D O I：

10.1016/j.fochx.2022.100321

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The chemical structure of GLP-1, a novel water-soluble heteropolysaccharide purified Ganoderma leucocontextum fruiting bodies, has been characterized in our previous study. This study aimed to investigate the immunostimulatory activity of GLP-1 in vitro and in vivo by using RAW264.7 macrophages and cyclophosphamide-induced immunosuppressed mice model. Results showed that GLP-1 was able to enhance phagocytic activity and promote the production of reactive oxygen species, nitric oxide, tumor necrosis factor-??, interleukin-6, and monocyte chemoattractant protein-1 in RAW264.7 macrophages. Moreover, GLP-1 could activate mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways through toll-like receptor 2 and dectin-1 receptors. Furthermore, GLP-1 increased the thymus index, serum immunoglobulin levels, and percentage of CD3+ T lymphocytes in cyclophosphamide-induced immunosuppressed mice. These results demonstrated that GLP-1 possessed significant immunostimulatory effects in vivo and in vitro and could be developed as an effective immunomodulator for application in functional foods.

引用

页数：10

共 40 条

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