Imatinib mesylate does not counteract ovarian tissue fibrosis in postnatal rat ovary

被引：8

作者：

Asadi-Azarbaijani, Babak ^{[1
]}

Braber, Saskia ^{[2
]}

van Duursen, Majorie ^{[3
]}

Jahnukainen, Kirsi ^{[4
,5
,6
]}

Santos, Regiane ^{[7
,8
]}

Oskam, Irma ^{[9
]}

机构：

[1] VID Specialized Univ, Fac Hlth Studies, PB 184, N-0319 Oslo, Norway

[2] Univ Utrecht, Fac Vet Med, IRAS, Utrecht, Netherlands

[3] Vrije Univ Amsterdam, Fac Sci, Dept Environm & Hlth, Amsterdam, Netherlands

[4] Univ Helsinki, New Childrens Hosp, Pediat Res Ctr, Helsinki, Finland

[5] Helsinki Univ Hosp, Helsinki, Finland

[6] NORDFERTIL Res Lab, Dept Womens & Childrens Hlth, Stockholm, Sweden

[7] Fed Univ Para, Lab Wild Anim Biol & Med, Castanhal, Para, Brazil

[8] Schothorst Feed Res, Lelystad, Netherlands

[9] Norwegian Univ Life Sci, Prod Anim Expt Res Ctr SHF, Oslo, Norway

来源：

REPRODUCTIVE BIOLOGY | 2019年 / 19卷 / 02期

关键词：

Imatinib; Tissue fibrosis; Ovary; FOXO1; FOXO3; RECEPTOR TYROSINE KINASES; C-KIT; TRANSCRIPTION FACTORS; MECHANISMS; ACTIVATION; INHIBITION; FOXO1; CHEMOTHERAPY; GROWTH; TRANSDIFFERENTIATION;

D O I：

10.1016/j.repbio.2019.03.003

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chemotherapy may result in ovarian atrophy, a depletion of the primordial follicle pool, diminished ovarian weight, cortical and stromal fibrosis. Imatinib mesylate is an anticancer agent that inhibits competitively several receptor tyrosine kinases (RTKs). RTKs play important roles in cell metabolism, proliferation, and apoptosis. In clinic, imatinib mesylate is also known as an anti-fibrotic medicine. In the present study, the impact of imatinib on the ovarian tissue was investigated by assessing ovarian tissue fibrosis in postnatal rat administered with or without imatinib for three days. Fibrosis in the ovarian tissue was determined by histology (Picrosirius and Masson's trichrome staining) and the protein expression of vimentin and alpha-smooth muscle actin (alpha-SMA). Furthermore, mRNA expression of Forkhead box transcription factor O1 and O3 (FOXO1 and FOXO3), which are markers of cell proliferation was quantified. A short-term exposure to imatinib showed to increase tissue fibrosis in ovaries. This was observed by Masson's trichrome staining. Exposure to imatinib led also to a down-regulation of vimentin protein expression and up-regulation mRNA expression of FOXO3. This may indicate a role of FOXO3 in ovarian tissue fibrosis in postnatal rat ovaries.

引用

页码：133 / 138

页数：6

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