Quantification of urine 17-ketosteroid sulfates and glucuronides by high-performance liquid chromatography-ion trap mass spectroscopy

被引：28

作者：

Jia, Q ^{[1
]}

Hong, MF ^{[1
]}

Pan, ZX ^{[1
]}

Orndorff, S ^{[1
]}

机构：

[1] Univera Pharmaceut Inc, Broomfield, CO 80021 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 750卷 / 01期

关键词：

17-ketosteroid sulfate; 17-ketosteroid glucuronide; steroids;

D O I：

10.1016/S0378-4347(00)00435-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurement of androgen steroids has been utilized as a clinical indicator of adrenal function, androgen abuse, and as a prediction of general health or biological aging. An improved high-performance liquid chromatography-ion trap mass spectroscopic method with sonic spray ionization (SSI) technology for the quantification of individual urinary 17-ketosteroid sulfates and glucuronides was developed and validated. Sample preparation was simplified using a C-18 cartridge followed by direct injection onto a reversed-phase HPLC column. Individual 17-ketosteroid from 63 urinary specimens collected in a 24-h period was measured. 17-Ketosteroid conjugates, total 17-KS-S and the ratio of total 17-KS-S to creatinine referred to herein as the Anabolic/Catabolic Index (ACI) showed statistically significant negative correlations with age. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：81 / 91

页数：11

共 26 条

[1] DEHYDROEPIANDROSTERONE-SULFATE (DHEAS) IN THE OLDEST-OLD, AGED 85 AND OVER [J].