A microsatellite genetic map of the turbot (Scophthalmus maximus)

被引:76
作者
Bouza, Carmen [1 ]
Hermida, Miguel [1 ]
Pardo, Belen G. [1 ]
Fernandez, Carlos [1 ]
Gonzalez Fortes, Gloria [1 ]
Castro, Jaime [1 ]
Sanchez, Laura [1 ]
Presa, Pablo [2 ]
Perez, Montse [2 ]
Sanjuan, Andres [2 ]
de Carlos, Alejandro [2 ]
Antonio Alvarez-Dios, Jose [3 ]
Ezcurra, Susana [4 ]
Cal, Rosa M. [5 ]
Piferrer, Francesc [6 ]
Martinez, Paulino [1 ]
机构
[1] Univ Santiago de Compostela, Fac Vet, Dept Genet, Lugo 27002, Spain
[2] Univ Vigo, Dept Biochem Genet & Immunol, Vigo 36310, Spain
[3] Univ Santiago de Compostela, Dept Appl Math, Santiago De Compostela 15782, Spain
[4] R&D Stolt Sea Farm, Carnola 15292, A Coruna, Spain
[5] Oceanog Ctr Vigo, Spanish Oceanog Inst, Vigo 36280, Spain
[6] Sci Res Major Ctr, Inst Marine Sci, Barcelona 08003, Spain
关键词
D O I
10.1534/genetics.107.075416
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites were mapped in 26 linkage groups, six markers remaining unlinked. The consensus map length was 1343.2 cM, with an averarge distance between markers of 6.5 +/- 0.5 cM. Similar length of female and male maps was evidenced. However, the mean recombination at common intervals throughout the genome revelaed significant differences between sexes, similar to 1.6 times higher in teh female than in the male. The comparison of turbot microsatellite flanking sequences against the Tetraodon nigroviridis genome revealed 55 significant matches, with a mean length of 102 bp and high sequence similarity (81-100%). The comparative mapping revealed significant syntenic regions among fish species. This study represents the first linkage map in the turbot, one of the most important flatfish in European aquaculture. This map will he suitable For QTL identification of productive traits in this species and for further evolutionary studies in fish and vertebrate species.
引用
收藏
页码:2457 / 2467
页数:11
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