CRISPR-Based Directed Evolution for Crop Improvement

被引：26

作者：

Butt, Haroon ^{[1
]}

Zaidi, Syed Shan-e-Ali ^{[2
]}

Hassan, Norhan ^{[1
]}

Mahfouz, Magdy ^{[1
]}

机构：

[1] King Abdullah Univ Sci & Technol, Lab Genome Engn & Synthet Biol, Thuwal 239556900, Saudi Arabia

[2] Univ Liege, Gembloux Agro BioTech, TERRA Res & Teaching Ctr, Plant Genet Lab, Passage Deportes 2, B-5030 Gembloux, Belgium

来源：

TRENDS IN BIOTECHNOLOGY | 2020年 / 38卷 / 03期

关键词：

TARGETED MUTAGENESIS;

D O I：

10.1016/j.tibtech.2019.08.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.

引用

页码：236 / 240

页数：6

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