Estimation of the effectiveness ratio (α/β) for resistant cancer cells in U87MG human glioblastoma

被引：2

作者：

Marmolejo-Leon, Perla ^{[1
,2
]}

Patricia Azorin-Vega, Erika ^{[1
]}

Jimenez-Mancilla, Nallely ^{[1
]}

Javier Mendoza-Nava, Hector ^{[1
]}

Mitsoura, Eleni ^{[2
]}

Pineda, Benjamin ^{[3
]}

Torres-Garcia, Eugenio ^{[2
]}

机构：

[1] Inst Natl Invest Nucl, Gerencia Aplicac Nucl Salud, Ocoyoacac 52750, Estado De Mexic, Mexico

[2] Univ Autonoma Estado Mexico, Fac Med, Toluca 50120, Estado De Mexic, Mexico

[3] SSA, Inst Nacl Neurol Manuel Velasco Suarez, Lab Neurooncol & Neuroinmunol, Mexico City 14269, DF, Mexico

来源：

APPLIED RADIATION AND ISOTOPES | 2018年 / 141卷

关键词：

Cancer stem cells; Glioblastoma; Linear quadratic model; Survival curves; STEM-CELLS; RADIOBIOLOGY; MULTIFORME;

D O I：

10.1016/j.apradiso.2018.01.011

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Glioblastoma contains self-renewing, tumorigenic cancer stem-like cells that contribute to tumor initiation and therapeutic resistance. The aim of this research was to estimate and compare the effectiveness ratio (alpha/beta) of stem-like cells and differentiated glioma cells derived from the U87MG glioblastoma cell line. Cell survival experiments were obtained in a dose range of 0-20 Gy (13.52 +/- 0.09 Gy/h) as a hyperfractionationated accelerated radiotherapy scheme. Biochemical characterization of the post-irradiated cells was performed by flow cytometry analysis and the percentage of stem-like cells that resisted irradiation was determined by the CD133 expression. Results showed that U87MG stem-like cells are highly proliferative and more radioresistant than the U87MG adherent group (with a lesser stem-like character), this in association with the calculated alpha/beta ratio of 17 and 14.1, respectively.

引用

页码：156 / 161

页数：6

共 28 条

[1] Cell cycle regulation of NF-κB-Binding activity in cells from human glioblastomas [J].

Ansari, SA ;

Safak, M ;

Del Valle, L ;

Enam, S ;

Amini, S ;

Khalili, K .

EXPERIMENTAL CELL RESEARCH, 2001, 265 (02) :221-233

[2] Glioma stem cells promote radioresistance by preferential activation of the DNA damage response [J].

Bao, Shideng ;

Wu, Qiulian ;

McLendon, Roger E. ;

Hao, Yueling ;

Shi, Qing ;

Hjelmeland, Anita B. ;

Dewhirst, Mark W. ;

Bigner, Darell D. ;

Rich, Jeremy N. .

NATURE, 2006, 444 (7120) :756-760

[3] Cancer stem cells from human glioma cell line are resistant to Fas-induced apoptosis [J].

Bertrand, Julian ;

Begaud-Grimaud, Gaelle ;

Bessette, Barbara ;

Verdier, Mireille ;

Battu, Serge ;

Jauberteau, Marie-Odile .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2009, 34 (03) :717-727

[4] Expression of CD133 and CD44 in glioblastoma stem cells correlates with cell proliferation, phenotype stability and intra-tumor heterogeneity [J].

Brown, Daniel V. ;

Filiz, Gulay ;

Daniel, Paul M. ;

Hollande, Frederic ;

Dworkin, Sebastian ;

Amiridis, Stephanie ;

Kountouri, Nicole ;

Ng, Wayne ;

Morokoff, Andrew P. ;

Mantamadiotis, Theo .

PLOS ONE, 2017, 12 (02)

[5] Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study [J].

Buch, Karl ;

Peters, Tanja ;

Nawroth, Thomas ;

Saenger, Markus ;

Schmidberger, Heinz ;

Langguth, Peter .

RADIATION ONCOLOGY, 2012, 7

[6] Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo [J].

Goodell, MA ;

Brose, K ;

Paradis, G ;

Conner, AS ;

Mulligan, RC .

JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (04) :1797-1806

[7] Notch1 Stimulation Induces a Vascularization Switch With Pericyte-Like Cell Differentiation of Glioblastoma Stem Cells [J].

Guichet, Pierre-Olivier ;

Guelfi, Sophie ;

Teigell, Marisa ;

Hoppe, Liesa ;

Bakalara, Norbert ;

Bauchet, Luc ;

Duffau, Hugues ;

Lamszus, Katrin ;

Rothhut, Bernard ;

Hugnot, Jean-Philippe .

STEM CELLS, 2015, 33 (01) :21-34

[8] The role of basic fibroblast growth factor in glioblastoma multiforme and glioblastoma stem cells and in their in vitro culture [J].

Haley, Elizabeth M. ;

Kim, Yonghyun .

CANCER LETTERS, 2014, 346 (01) :1-5

[9] Cancer stem cells: From bench to bedside [J].

Jones, Richard J. ;

Matsui, William .

BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2007, 13 (01) :47-52

[10] Persistence of a small subpopulation of cancer stem-like cells in the C6 glioma cell line [J].

Kondo, T ;

Setoguchi, T ;

Taga, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (03) :781-786

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