Model phase separations of proteins using aqueous/ethanol components

被引：51

作者：

Louwrier, A ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 05期

关键词：

D O I：

10.1023/A:1008818229903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Of several saturated or partially saturated inorganic salt solutions forming stable biphasic systems when mixed with ethanol, K2HPO4/ethanol was used to partition several model proteins, including lysozyme, BSA, chymotrypsinogen and the DNA polymerase from Thermus aquaticus. Partitioning was pH-dependent, and was successfully used for protein isolation from a mixture. Varying degrees of enzymatic activities were maintained.

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页码：363 / 365

页数：3

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