Amidrazone modified acrylic fabric activated with cyanuric chloride: A novel and efficient support for horseradish peroxidase immobilization and phenol removal

被引：60

作者：

Almulaiky, Yaaser Q. ^{[1
,2
]}

EI-Shishtawy, Reda M. ^{[3
,4
]}

Aldhahri, Musab ^{[5
,6
]}

Mohamed, Saleh A. ^{[7
]}

Afifi, Mohamed ^{[8
,9
]}

Abdulaal, Wesam H. ^{[5
]}

Mahyoub, Jazem A. ^{[10
]}

机构：

[1] Univ Jeddah, Fac Sci & Arts, Chem Dept, POB 355, Khulais 21921, Khulais, Saudi Arabia

[2] Taiz Univ, Fac Appl Sci, Chem Dept, Taizi, Yemen

[3] King Abdulaziz Univ, Fac Sci, Chem Dept, POB 80200, Jeddah 21589, Saudi Arabia

[4] Natl Res Ctr, Text Res Div, Dyeing Printing & Text Auxiliaries Dept, Cairo 71516, Egypt

[5] King Abdulaziz Univ, Fac Sci, Dept Biochem, POB 80200, Jeddah 21589, Saudi Arabia

[6] King Abdulaziz Univ, Ctr Nanotechnol, Jeddah 21589, Saudi Arabia

[7] Natl Res Ctr, Mol Biol Dept, Cairo, Egypt

[8] Univ Jeddah, Fac Sci, Dept Biochem, POB 80203, Jeddah 21589, Saudi Arabia

[9] Zagazig Univ, Fac Vet Med, Biochem Dept, Zagazig, Egypt

[10] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, POB 80203, Jeddah 21589, Saudi Arabia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 140卷

关键词：

Horseradish peroxidase; Immobilization; Hydrazine hydrochloride; COVALENT IMMOBILIZATION; ELECTROSPUN NANOFIBERS; ALPHA-AMYLASE; ENZYME; NANOPARTICLES; BIOSENSOR; MEMBRANES; MATRIX; BIODEGRADATION; STABILIZATION;

D O I：

10.1016/j.ijbiomac.2019.08.179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, hydrazine treated acrylic fabrics (polyacrylonitrile, PAN) activated with cyanuric chloride was developed as supporting material for horseradish peroxidase (HRP) immobilization. The immobilization of HRP onto the modified supporting material was achieved after being end-over-end incubated for 12 h. Field emission scanning electron microscopy and Fourier-transform infrared spectroscopy techniques were used to confirm the successful immobilization. Reusability experiment was performed to estimate the ability of the immobilized HRP to recover the reaction medium, in which it was observed to retain 78% of its original activity after 10 cycles. Relative to the soluble HRP, the optimum pH and temperature for the immobilized HRP were shifted to 7-7.5 and 50 degrees C, respectively. The kinetic parameters of guaiacol and H2O2 for the immobilized HRP were determined to be Km/Vmax = 57.61, 11.35 and Kcat/Km = 1.87, 1.86, respectively, while the values for the free form were Km/Vmax = 41.49, 6.23 and Kcat/Km = 1.87, 1.86, respectively. Compared to the soluble form, the immobilized HRP exhibited higher resistance toward metal ions and some organic solvents. For an application perspective. The immobilization of HRP using this procedure has the potential to be used for industrial application and waste-water treatment. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：949 / 958

页数：10

共 61 条

[61] Heterojunction of g-C3N4/BiOI Immobilized on Flexible Electrospun Polyacrylonitrile Nanofibers: Facile Preparation and Enhanced Visible Photocatalytic Activity for Floating Photocatalysis [J].

Zhou, Xuejiao ;

Shao, Changlu ;

Yang, Shu ;

Li, Xiaowei ;

Guo, Xiaohui ;

Wang, Xiaoxiao ;

Li, Xinghua ;

Liu, Yichun .

ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 2018, 6 (02) :2316-2323

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