A time-resolved fluoroimmunoassay to assay the rabies virus glycoprotein: application for estimation of human rabies vaccine potency

被引：24

作者：

Lin, Guanfeng ^{[1
]}

Chen, Shaolang ^{[2
]}

Zhao, Hui ^{[3
]}

Liang, Junyu ^{[2
]}

Deng, Qiaoting ^{[2
]}

Liang, Rongliang ^{[2
]}

Guo, Xinxin ^{[2
]}

Chen, Zhenhua ^{[2
]}

Chen, Baihong ^{[1
]}

Liu, Tiancai ^{[2
,4
]}

Wu, Yingsong ^{[2
,4
]}

机构：

[1] Southern Med Univ, Sch Lab Med & Biotechnol, Teaching & Sci Res Ctr, Guangzhou, Guangdong, Peoples R China

[2] Southern Med Univ, Inst Antibody Engn, Sch Lab Med & Biotechnol, Guangzhou, Guangdong, Peoples R China

[3] Southern Med Univ, Sch Basic Med Sci, Dept Human Anat, Guangzhou, Guangdong, Peoples R China

[4] Southern Med Univ Guangzhou, Sch Publ Hlth, Guangdong Prov Key Lab Trop Dis Res, Guangzhou, Guangdong, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

CYTOKERATIN; 19; FRAGMENT; CARCINOEMBRYONIC ANTIGEN; MONOCLONAL-ANTIBODY; HUMAN SERUM; QUANTITATION; IMMUNOASSAY; LABEL; ELISA; STANDARDIZATION; NUCLEOPROTEIN;

D O I：

10.1038/s41598-017-07687-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Replacement of the in vivo rabies vaccine potency test (NIH test) by in vitro methods had been discussed by several researcher including WHO expert working groups. In this paper, a time-resolved fluoroimmunoassay (TRFIA) for the assay of rabies virus glycoprotein in rabies vaccine was first established to estimate the rabies vaccine potency by using specific monoclonal antibody that only recognized the native, trimeric and immunogenic form of rabies virus glycoprotein. Potency of the rabies virus glycoprotein was assayed with satisfactory performance under optimal conditions, and the method demonstrated satisfactory results when applied in practical samples. The correlation coefficient of potency values obtained from the present TRFIA and ELISA was 0.912, and 0.903 for those from the present TRFIA and NIH test. These preliminary results confirmed that this TRFIA can replace ELISA with higher performance, and could be a promising replacement of the NIH test. Based upon these results, the present TRFIA seemed to be a convenient tool for evaluating rabies vaccine potency and its products at different stages accordingly.

引用

页数：9

共 36 条

[1] NIH TEST, A PROBLEMATIC METHOD FOR TESTING POTENCY OF INACTIVATED RABIES VACCINE [J].