Proteomic analysis of activity-dependent synaptic plasticity in hippocampal neurons

被引:41
作者
Piccoli, Giovanni
Verpelli, Chiara
Tonna, Noemi
Romorini, Stefano
Allessio, Massimo
Nairn, Angus C.
Bachi, Angela
Sala, Carlo
机构
[1] Univ Milan, CNR, Inst Neurosci, I-20129 Milan, Italy
[2] Univ Milan, Dept Pharmacol, I-20129 Milan, Italy
[3] DIBIT San Raffaele Sci Inst, Proteome Biochem Unit, Milan, Italy
[4] Yale Univ, Sch Med, Dept Psychiat, New Haven, CT 06508 USA
关键词
excitatory synapses; phosphorylation; dendritic spines; neuroproteomic analysis; mass spectrometry;
D O I
10.1021/pr0701308
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Following long-term treatment with bicuculline and tetroclotoxin (TTX) aimed at modifying synaptic activity in cultured neurons, we used a proteomic approach to identify the associated changes in protein expression. The neurons were left untreated, or treated with bicuculline or TTX, and fractionated by means of differential detergent extraction, after which the proteins in each fraction were separated by means of two-dimensional (2D) gel electrophoresis, and 57 proteins of interest were identified by mass spectrometry. The proteins that showed altered expression and/or post-translational modifications include proteins or enzymes involved in regulating cell and protein metabolism, the cytoskeleton, or mitochondrial activity. These results suggest that extensive alterations in neuronal protein expression take place as a result of increased or decreased synaptic activity.
引用
收藏
页码:3203 / 3215
页数:13
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