Improving gold nanorod delivery to the central nervous system by conjugation to the shuttle Angiopep-2

被引：39

作者：

Velasco-Aguirre, Carolina ^{[1
]}

Morales-Zavala, Francisco ^{[1
]}

Salas-Huenuleo, Edison ^{[1
]}

Gallardo-Toledo, Eduardo ^{[1
]}

Andonie, Oscar ^{[2
]}

Munoz, Luis ^{[2
]}

Rojas, Ximena ^{[2
]}

Acosta, Gerardo ^{[3
,4
]}

Sanchez-Navarro, Macarena ^{[5
]}

Giralt, Ernest ^{[3
]}

Araya, Eyleen ^{[6
]}

Albericio, Fernando ^{[3
,4
,7
]}

Javier Kogan, Marcelo ^{[1
]}

机构：

[1] Univ Chile, Adv Ctr Chron Dis ACCDiS, Fac Ciencias Quim & Farmaceut, Dept Quim Farmacol & Toxicol, Santiago, Chile

[2] CCHEN, Comis Chilena Energia Nucl, Secc Metrol Quim, Nueva Bilbao 12501, Santiago, Chile

[3] Univ Barcelona, Dept Inorgan & Organ Chem, Marti i Franques 1, E-08028 Barcelona, Spain

[4] CIBER BBN, Networking Ctr Bioengn Biomat & Nanomed, Barcelona Sci Pk, Barcelona 08028, Spain

[5] Barcelona Inst Sci & Technol, Inst Res Biomed IRB Barcelona, Baldiri Reixac 10, Barcelona 08028, Spain

[6] Univ Andres Bello, Fac Ciencias Exactas, Dept Ciencias Quim, Republica 275, Santiago 8370146, Chile

[7] Univ KwaZulu Natal, Sch Chem & Phys, ZA-4001 Durban, South Africa

来源：

NANOMEDICINE | 2017年 / 12卷 / 20期

关键词：

angiopep-2; blood-brain barrier; drug delivery; gold nanoparticle; gold nanorods; photothermal effect; theranostics; BRAIN DELIVERY; POLY(ETHYLENE GLYCOL); NANOPARTICLES; PEPTIDE; PROTEIN; DRUG; DOXORUBICIN; BIODISTRIBUTION; STABILITY; TRANSPORT;

D O I：

10.2217/nnm-2017-0181

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: To improve the in vivo delivery of gold nanorods (GNRs) to the central nervous system of rats, these gold nanoparticles were conjugated to Angiopep-2, a shuttle peptide that can cross the blood-brain barrier. Materials & methods: GNRs were synthesized and modified using polyethylene glycol and Angiopep-2 (GNR-PEG-Angiopep-2). The physicochemical properties, in vitro cytotoxicity and ex vivo biodistribution of the conjugate were examined. Results: GNR-PEG-Angiopep-2 was stable over the following days, and the different concentrations that were tested did not affect the viability of microvascular endothelial cells. The conjugation of Angiopep-2 to GNRs enhanced the endocytosis of these particles (in vitro) and the accumulation in brains (in vivo), when compared with GNRs modified only with PEG. Conclusion: This study provides evidence that Angiopep-2 improves the delivery of GNRs to the brain parenchyma. This property is highly relevant for future applications of GNRs as platforms for photothermal and theranostic purposes.

引用

页码：2503 / 2517

页数：15