Aspartate aminotransferase is involved in cold adaptation in psychrophilic Pseudomonas syringae

被引：13

作者：

Sundareswaran, V. R. ^{[1
]}

Singh, Ashish Kumar ^{[1
]}

Dube, Smita ^{[1
]}

Shivaji, S. ^{[1
]}

机构：

[1] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

ARCHIVES OF MICROBIOLOGY | 2010年 / 192卷 / 08期

关键词：

Pseudomonas syringae; Psychrophilic bacterium; Aspartate aminotransferase; Transposon mutagenesis; Cold-inducible gene; LOW-TEMPERATURE; ANTARCTIC BACTERIUM; GROWTH; PROTEIN; RNA; EXPRESSION; GENES; IDENTIFICATION; CLONING; OPERON;

D O I：

10.1007/s00203-010-0591-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28A degrees C; but at 4A degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was transiently enhanced when cells were shifted from 22 to 4A degrees C indicating that AAT is cold-inducible. Complementation of the mutated AAT transformed CSM2 from a cold-sensitive phenotype to a cold-resistant phenotype like the wild-type cells, thus providing evidence for the first time that AAT is required for low-temperature growth.

引用

页码：663 / 672

页数：10

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