Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway

被引：9

作者：

Ha, Jung Min ^{[1
]}

Baek, Seung Hoon ^{[2
]}

Kim, Young Hwan ^{[1
]}

Jin, Seo Yeon ^{[1
]}

Lee, Hye Sun ^{[1
]}

Kim, Sun Ja ^{[3
]}

Shin, Hwa Kyoung ^{[4
]}

Lee, Dong Hyung ^{[5
]}

Song, Sang Heon ^{[6
]}

Kim, Chi Dae ^{[1
]}

Bae, Sun Sik ^{[1
]}

机构：

[1] Pusan Natl Univ, Sch Med, Gene & Cell Therapy Ctr Vessel Associated Dis, Dept Pharmacol,Med Res Inst, Yangsan, South Korea

[2] Pusan Natl Univ Hosp, Dept Anesthesia & Pain Med, Yangsan, South Korea

[3] Dong A Univ, Dept Phys, Busan, South Korea

[4] Pusan Natl Univ Korean Med, Dept Anat, Yangsan, South Korea

[5] Pusan Natl Univ Hosp, Dept Obstet & Gynecol, Yangsan, South Korea

[6] Pusan Natl Univ Hosp, Dept Internal Med, Busan, South Korea

来源：

EXPERIMENTAL AND MOLECULAR MEDICINE | 2016年 / 48卷

基金：

新加坡国家研究基金会;

关键词：

VEGF-MEDIATED ANGIOGENESIS; ENDOTHELIAL GROWTH-FACTOR; VASCULAR DEVELOPMENT; C ISOZYMES; IN-VITRO; PDGF-B; CELLS; MORPHOGENESIS; ACTIVATION; EXPRESSION;

D O I：

10.1038/emm.2016.39

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-beta 3 (PLC-beta 3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-beta 3 in human umbilical vein endothelial cells (HUVECs) significantly delayed proliferation, migration and capillary-like tube formation. In addition, mice lacking PLC-beta 3 showed impaired retinal angiogenesis with delayed endothelial proliferation, reduced endothelial cell activation, abnormal vessel formation and hemorrhage. Finally, tumor formation was significantly reduced in mice lacking PLC-beta 3 and showed irregular size and shape of blood vessels. These results suggest that regulation of endothelial function by PLC-beta 3 may contribute to angiogenesis.

引用

页码：e240 / e240

页数：8

共 45 条

[1] Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors [J].