Identification of LIG1 and LIG3 as prognostic biomarkers in breast cancer

DNA ligase (LIG) plays a key role in connecting the 3 '-OH end of a DNA strand to the 5 '-P end of another DNA strand, resulting in the formation of a phosphodie-ster bond. It has been reported that LIGs (including LIG1, LIG3 and LIG4) play important roles in the occurrence and progression of many cancers. However, the role of LIGs in breast cancer (BC) is still unclear. In this study, we aim to reveal the expression level, function, and prog-nostic value of LIGs in BC. Bioinformatic tools were used to study the expression level, potential function and prognostic value of LIG1 and LIG3 in BC patients. ENCORI was used to predict microRNAs (miRNAs) that regulate LIG1 and LIG3 and established a valuable miRNA-mRNA regula-tion network for BC. We found that the expression of LIG1 and LIG3 was upregulated in BC and predicted high relapse-free survival (RFS) in BC patients. Functional annotation analysis was performed to reveal the role of LIG1 and LIG3 in BC. In addition, hsa-miR-22-3p was identified to be potentially involved in the regulation of LIG3. We suggest that LIG1 and LIG3 are novel valuable prognostic biomar-kers for BC and has-miRNA-22-3p may be a potential ther-apeutic target for BC.