Up-Regulation of Na+/Ca2+ Exchange in Megakaryocytes Following TGFβ1 Treatment

被引：8
作者：
Almilaji, Ahmad [1 ]
Yan, Jing [1 ,2 ]
Hosseinzadeh, Zohreh [1 ,4 ]
Schmid, Evi [1 ,3 ]
Gawaz, Meinrad [1 ]
Lang, Florian [1 ]
机构：
[1] Univ Tubingen, Dept Cardiol Vasc Med & Physiol, Tubingen, Germany
[2] Xinxiang Med Univ, Dept Physiol, Xinxiang, Peoples R China
[3] Univ Childrens Hosp Tuebingen, Dept Pediat Surg & Pediat Urol, Tubingen, Germany
[4] Inst Ophthalm Res, Ctr Ophthalmol, Expt Retinal Prosthet Grp, Tubingen, Germany
来源：
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2016年 / 39卷 / 02期
关键词：
Megakaryocytes; Patch clamp; Cytosolic Ca2+; NCKX; NCX; PLATELET ACTIVATION; DENDRITIC CELLS; CALCIUM INFLUX; CA2+ ENTRY; INHIBITION; DEATH; ORAI1; INFLAMMATION; CHANNELS; MUTATION;
D O I：
10.1159/000445660
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Background: Blood platelets are activated by increase of cytosolic Ca2+ activity ([Ca2+](i)). Ca2+ entry is accomplished in part by store operated Ca2+ entry (SOCE) involving Ca2+ release activated Ca2+-channel (CRAC) moiety rail and its regulator STIM1, which are stimulated by depletion of intracellular Ca2+ stores. An increase of [Ca2+], is terminated byexchange. The expression of both, Orail and STIM1 in megakaryocytes is up-regulated by tumor growth factor TGF beta 1, a powerful regulator of megakaryocyte differentiation. The present study explored whether TGF beta 1 similarly modifies megakaryocyte Na+/Ca2+- exchanger activity. Methods: [Ca2+](i), was determined utlizing Fura 2 fluorescence, SOCE from increase of [Call, following readdition of extracellular Ca2+ after store depletion, and Na+/Ca(2+-)exchanger activity from increase of [Ca2+](i), and whole cell currents following removal of extracellular Na+. Results: TGF beta 1 treatment not only augments the increase of [Ca2+](i) following store depletion and SOCE, but significantly up-regulates Na+/Ca2+- exchanger activity as apparent from [Call measurements and whole cell currents. Conclusions: TGF beta 1 is a powerful stimulator of both, SOCE and Na+/Ca2+- exchanger activity in megakaryocytes. (C) 2016 The Author(s) Published by S. Karger AG, Basel
引用
收藏
页码：693 / 699
页数：7
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