Antimicrobial compounds from endophytic Streptomyces sp BCC72023 isolated from rice (Oryza sativa L.)

被引：30

作者：

Supong, Khomsan ^{[1
,2
]}

Thawai, Chitti ^{[1
,5
]}

Choowong, Wilunda ^{[3
]}

Kittiwongwattana, Chokchai ^{[1
,5
]}

Thanaboripat, Dusanee ^{[1
]}

Laosinwattana, Chamroon ^{[4
]}

Koohakan, Prommart ^{[4
]}

Parinthawong, Nonglak ^{[4
]}

Pittayakhajonwut, Pattama ^{[3
]}

机构：

[1] King Mongkuts Inst Technol Ladkrabang, Fac Sci, Dept Biol, Bangkok 10520, Thailand

[2] Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem & Microbiol, Bangkok 10330, Thailand

[3] NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Thailand Sci Pk,Paholyothin Rd, Pathum Thani 12120, Thailand

[4] King Mongkuts Inst Technol Ladkrabang, Fac Agr Technol, Dept Plant Prod Technol, Bangkok 10520, Thailand

[5] King Mongkuts Inst Technol Ladkrabang, Fac Sci, Actinobacterial Res Unit, Bangkok 10520, Thailand

来源：

RESEARCH IN MICROBIOLOGY | 2016年 / 167卷 / 04期

关键词：

Antimicrobial; Endophytic actinomycete; Streptomyces; Secondary metabolites; Macrolides; Efomycins; MULTIPLE SEQUENCE ALIGNMENT; SP NOV; IDENTIFICATION; MACROLIDES; ACTINOBACTERIA; RESAZURIN;

D O I：

10.1016/j.resmic.2016.01.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An endophytic actinomycete strain BCC72023 was isolated from rice (Oryza sativa L.) and identified as the genus Streptomyces, based on phenotypic, chemotaxonomic and 16S rRNA gene sequence analyses. The strain showed 99.80% similarity compared with Streptomyces samsunensis M1463(T). Chemical investigation led to the isolation of three macrolides, efomycins M (1), G (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-O-methylabierixin (5). To our knowledge, this is the first report of efomycin M being isolated from a natural source. The compounds were identified using spectroscopic techniques and comparison with previously published data. All compounds exhibited antimalarial activity against the Plasmodium falciparum, K-1 strain, a multidrug-resistant strain, with IC50 values in a range of 1.40-5.23 mu g/ml. In addition, these compounds were evaluated for biological activity against Mycobacterium tuberculosis, Bacillus cereus, Colletotrichum gloeosporioides and Colletotrichuni capsici, as well as cytotoxicity against both cancerous (MCF-7, KB, NCI -H187) and non-cancerous (Vero) cells. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：290 / 298

页数：9

共 55 条

[1] Streptomyces malaysiensis sp nov., a new streptomycete species with rugose, ornamented spores [J].