In cardiac rehabilitation, different endurance exercises such as walking and cycling are often performed. The training intensity for these modes is determined from a single treadmill or bicycle test by ventilatory threshold ( VT). In this study, differences of VT between walking and cycling and agreement of VT between training modes were assessed in cardiac patients. A total of 46 cardiac rehabilitation patients ( mean age 59.5 +/- 8.4 years, 45 males) ( 31 untrained and 15 trained) completed a maximal exercise test on bicycle and treadmill, with breath-by-breath analysis of oxygen uptake (VO2), carbon dioxide production and expiratory volume. VT was determined by V-slope method. Correlations of VT and VO2peak were calculated between exercise modes. Bland-Altman plots were made for determining VT agreement between modes. VT was significantly different between walking and cycling in trained patients ( P < 0.05), but not in untrained patients ( P > 0.05). When untrained and trained patients were compared, VT correlation was lower (r = 0.50) in the former group, as compared to the latter group ( r = 0.78). Also, Bland-Altman plots showed smaller limits of agreement for VT in trained (2 SD - 1.6 to 7.8 ml/min/kg), as compared to untrained patients ( 2 SD - 7.0 to 9.6 ml/min/kg). In trained patients, VT correlates well between training methods, but is highly exercise mode specific. In untrained patients, VT is not exercise mode specific, but the VT has a low correlation between training modes. This study shows that VT should be assessed by the appropriate exercise model for determining exercise intensity in cardiac rehabilitation.