Suppression of Presynaptic Glutamate Release by Postsynaptic Metabotropic NMDA Receptor Signalling t o Pannexin-1

被引:47
作者
Bialecki, Jennifer [1 ]
Werner, Allison [1 ]
Weilinger, Nicholas L. [1 ]
Tucker, Catharine M. [1 ]
Vecchiarelli, Haley A. [1 ]
Egana, Jon [2 ]
Mendizabal-Zubiaga, Juan [2 ]
Grandes, Pedro [2 ]
Hill, Matthew N. [1 ]
Thompson, Roger J. [1 ]
机构
[1] Univ Calgary, Dept Cell Biol & Anat, Hotchkiss Brain Inst, Calgary, AB T2N 4N1, Canada
[2] Univ Basque Country UPV EHU, Dept Neurosci, Leioa 48940, Spain
基金
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词
anandamide; glutamate transmission; hippocampus; metabotropic NMDAR; parmexin; TRPV1; LONG-TERM DEPRESSION; TRPV1; ACTIVATION; CHANNEL; ANANDAMIDE; FAMILY; CELLS; MODULATION; MEMBRANE; PROTEIN; DESENSITIZATION;
D O I
10.1523/JNEUROSCI.0257-19.2019
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The impact of pannexin-1 (Panx1) channels on synaptic transmission is poorly understood. Here, we show that selective block of Panx1 in single postsynaptic hippocampal CA1 neurons from male rat or mouse brain slices causes intermittent, seconds long increases in the frequency of sEPSC following Schaffer collateral stimulation. The increase in sEPSC frequency occurred without an effect on evoked neurotransmission. Consistent with a presynaptic origin of the augmented glutamate release, the increased sEPSC frequency was prevented by bath-applied EGTA-AM or TTX. Manipulation of a previously described metabotropic NMDAR pathway (i.e., by preventing ligand binding to NMDARs with competitive antagonists or blocking downstream Src kinase) also increased sEPSC frequency similar to that seen when Panx1 was blocked. This facilitated glutamate release was absent in transient receptor potential vanilloid 1 (TRPV1) KO mice and prevented by the TRPV1 antagonist, capsazepine, suggesting it required presynaptic TRPV1. We show presynaptic expression of TRPV1 by immunoelectron microscopy and link TRPV1 to Panx1 because Panx1 block increases tissue levels of the endovanilloid, anandamide. Together, these findings demonstrate an unexpected role for metabotropic NMDARs and postsynaptic Panx1 in suppression of facilitated glutamate neurotransmission.
引用
收藏
页码:729 / 742
页数:14
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