Solvatochromic studies on reversed phase liquid chromatographic phases .2. Characterization of stationary and mobile phases

被引：33
|
作者：
Lu, HY [1 ]
Rutan, SC [1 ]
机构：
[1] VIRGINIA COMMONWEALTH UNIV,DEPT CHEM,RICHMOND,VA 23284
来源：
ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 08期
关键词：
D O I：
10.1021/ac9507810
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
UV-visible spectra of three solvatochromic dyes, 4,6-dichloro-2-[2-(1-methyl-4-pyridinio)vinyl]phenolate, N-methyl-2-nitroaniline, and 2-nitroaniline, are employed to characterize the octadecyl-derivatized silica (ODS2) stationary phase and the corresponding mobile phases used in reversed-phase liquid chromatography. The mobile phases studied are methanol-water, acetonitrile-water, and tetrahydrofuran-water, with composition ranges from 100% to 5% of organic modifier. Across the whole mobile phase composition range, the ODS2 stationary phase shows three distinct behaviors upon variation of the mobile phase composition, which are described as non-retentive, solvated, and collapsed.
引用
收藏
页码：1387 / 1393
页数：7
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