CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death

被引:110
作者
Levy, Yves [1 ,2 ]
Wiedemann, Aurelie [1 ]
Hejblum, Boris P. [1 ,3 ]
Durand, Melany [1 ,3 ]
Lefebvre, Cecile [1 ]
Surenaud, Mathieu [1 ]
Lacabaratz, Christine [1 ]
Perreau, Matthieu [4 ]
Foucat, Emile [1 ]
Dechenaud, Marie [1 ]
Tisserand, Pascaline [1 ]
Blengio, Fabiola [1 ]
Hivert, Benjamin [3 ]
Gauthier, Marine [3 ]
Cervantes-Gonzalez, Minerva [5 ,6 ,7 ]
Bachelet, Delphine [5 ,7 ]
Laouenan, Cedric [5 ,7 ]
Bouadma, Lila [8 ]
Timsit, Jean-Francois [8 ]
Yazdanpanah, Yazdan [6 ,7 ]
Pantaleo, Giuseppe [1 ,4 ,9 ]
Hocini, Hakim [1 ]
Thiebaut, Rodolphe [1 ,3 ,10 ]
机构
[1] Univ Paris Est Creteil, Hop Henri Mondor, Vaccine Res Inst, Fac Med,INSERM U955,Team 16, 51 Av Marechal Lattre Tassigny, F-94010 Creteil, France
[2] AP HP, Grp Henri Mondor Albert Chenevier, Serv Immunol Clin, Creteil, France
[3] Univ Bordeaux, INSERM U1219 Bordeaux Populat Hlth Res Ctr, Dept Publ Hlth, Inria SISTM,UMR 1219, 146 Rue Leo Saignat, F-33076 Bordeaux, France
[4] Univ Lausanne, Lausanne Univ Hosp, Swiss Vaccine Res Inst, Lausanne, Switzerland
[5] Hop Bichat Claude Bernard, AP HP, INSERM, Ctr Invest Clin Epidemiol Clin 1425,Dept Epidemio, F-75018 Paris, France
[6] Hop Bichat Claude Bernard, AP HP, Serv Malad Infect & Trop, F-75018 Paris, France
[7] Univ Paris, INSERM, IAME UMR 1137, F-75018 Paris, France
[8] Hop Bichat Claude Bernard, AP HP, Med Intens & Reanimat Malad Infect, Paris, France
[9] Univ Lausanne, Lausanne Univ Hosp, Dept Med, Immunol & Allergy Serv, Lausanne, Switzerland
[10] CHU Bordeaux, Pole Sante Publ, Serv Informat Med, Bordeaux, France
基金
比尔及梅琳达.盖茨基金会;
关键词
COVID-19; INFECTION; CYTOKINE; MILD;
D O I
10.1016/j.isci.2021.102711
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The identification of patients with coronavirus disease 2019 and high risk of severe disease is a challenge in routine care. We performed cell phenotypic, serum, and RNA sequencing gene expression analyses in severe hospitalized patients (n = 61). Relative to healthy donors, results showed abnormalities of 27 cell populations and an elevation of 42 cytokines, neutrophil chemo-attractants, and inflammatory components in patients. Supervised and unsupervised analyses revealed a high abundance of CD177, a specific neutrophil activation marker, contributing to the clustering of severe patients. Gene abundance correlated with high serum levels of CD177 in severe patients. Higher levels were confirmed in a second cohort and in intensive care unit (ICU) than non-ICU patients (P < 0.001). Longitudinal measurements discriminated between patients with the worst prognosis, leading to death, and those who recovered (P = 0.01). These results highlight neutrophil activation as a hallmark of severe disease and CD177 assessment as a reliable prognostic marker for routine care.
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页数:20
相关论文
共 65 条
[1]   Sources of Type I Interferons in Infectious Immunity: Plasmacytoid Dendritic Cells Not Always in the Driver's Seat [J].
Ali, Shafaqat ;
Mann-Nuettel, Ritu ;
Schulze, Anja ;
Richter, Lisa ;
Alferink, Judith ;
Scheu, Stefanie .
FRONTIERS IN IMMUNOLOGY, 2019, 10
[2]   Multi-omics profiling of mouse gastrulation at single-cell resolution [J].
Argelaguet, Ricard ;
Clark, Stephen J. ;
Mohammed, Hisham ;
Stapel, L. Carine ;
Krueger, Christel ;
Kapourani, Chantriolnt-Andreas ;
Imaz-Rosshandler, Ivan ;
Lohoff, Tim ;
Xiang, Yunlong ;
Hanna, Courtney W. ;
Smallwood, Sebastien ;
Ibarra-Soria, Ximena ;
Buettner, Florian ;
Sanguinetti, Guido ;
Xie, Wei ;
Krueger, Felix ;
Gottgens, Berthold ;
Rugg-Gunn, Peter J. ;
Kelsey, Gavin ;
Dean, Wendy ;
Nichols, Jennifer ;
Stegle, Oliver ;
Marioni, John C. ;
Reik, Wolf .
NATURE, 2019, 576 (7787) :487-+
[3]   Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans [J].
Arunachalam, Prabhu S. ;
Wimmers, Florian ;
Mok, Chris Ka Pun ;
Perera, Ranawaka A. P. M. ;
Scott, Madeleine ;
Hagan, Thomas ;
Sigal, Natalia ;
Feng, Yupeng ;
Bristow, Laurel ;
Tsang, Owen Tak-Yin ;
Wagh, Dhananjay ;
Coller, John ;
Pellegrini, Kathryn L. ;
Kazmin, Dmitri ;
Alaaeddine, Ghina ;
Leung, Wai Shing ;
Chan, Jacky Man Chun ;
Chik, Thomas Shiu Hong ;
Choi, Chris Yau Chung ;
Huerta, Christopher ;
McCullough, Michele Paine ;
Lv, Huibin ;
Anderson, Evan ;
Edupuganti, Srilatha ;
Upadhyay, Amit A. ;
Bosinger, Steve E. ;
Maecker, Holden Terry ;
Khatri, Purvesh ;
Rouphael, Nadine ;
Peiris, Malik ;
Pulendran, Bali .
SCIENCE, 2020, 369 (6508) :1210-+
[4]   Disease severity-specific neutrophil signatures in blood transcriptomes stratify COVID-19 patients [J].
Aschenbrenner, Anna C. ;
Mouktaroudi, Maria ;
Kraemer, Benjamin ;
Oestreich, Marie ;
Antonakos, Nikolaos ;
Nuesch-Germano, Melanie ;
Gkizeli, Konstantina ;
Bonaguro, Lorenzo ;
Reusch, Nico ;
Basser, Kevin ;
Saridaki, Maria ;
Knoll, Rainer ;
Pecht, Tal ;
Kapellos, Theodore S. ;
Doulou, Sarandia ;
Kroeger, Charlotte ;
Herbert, Miriam ;
Holsten, Lisa ;
Horne, Arik ;
Gemuend, Ioanna D. ;
Rovina, Nikoletta ;
Agrawal, Shobhit ;
Dahm, Kilian ;
van Uelft, Martina ;
Drews, Anna ;
Lenkeit, Lena ;
Bruse, Niklas ;
Gerretsen, Jelle ;
Gierlich, Jannik ;
Becker, Matthias ;
Haendler, Kristian ;
Kraut, Michael ;
Theis, Heidi ;
Mengiste, Simachew ;
De Domenico, Elena ;
Schulte-Schrepping, Jonas ;
Seep, Lea ;
Raabe, Jan ;
Hoffmeister, Christoph ;
ToVinh, Michael ;
Keitel, Verena ;
Rieke, Gereon ;
Talevi, Valentina ;
Skowasch, Dirk ;
Aziz, N. Ahmad ;
Pickkers, Peter ;
van de Veerdonk, Frank L. ;
Netea, Mihai G. ;
Schultze, Joachim L. ;
Kox, Matthijs .
GENOME MEDICINE, 2021, 13 (01)
[5]  
Barnes CO, 2020, CELL, V182, P828, DOI [10.1016/j.cell.2020.06.025, 10.1101/2020.05.28.121533]
[6]   Autoantibodies against type I IFNs in patients with life-threatening COVID-19 [J].
Bastard, Paul ;
Rosen, Lindsey B. ;
Zhang, Qian ;
Michailidis, Eleftherios ;
Hoffmann, Hans-Heinrich ;
Zhang, Yu ;
Dorgham, Karim ;
Philippot, Quentin ;
Rosain, Jeremie ;
Beziat, Vivien ;
Manry, Jeremy ;
Shaw, Elana ;
Haljasmagi, Liis ;
Peterson, Part ;
Lorenzo, Lazaro ;
Bizien, Lucy ;
Trouillet-Assant, Sophie ;
Dobbs, Kerry ;
de Jesus, Adriana Almeida ;
Belot, Alexandre ;
Kallaste, Anne ;
Catherinot, Emilie ;
Tandjaoui-Lambiotte, Yacine ;
Le Pen, Jeremie ;
Kerner, Gaspard ;
Bigio, Benedetta ;
Seeleuthner, Yoann ;
Yang, Rui ;
Bolze, Alexandre ;
Spaan, Andras N. ;
Delmonte, Ottavia M. ;
Abers, Michael S. ;
Aiuti, Alessandro ;
Casari, Giorgio ;
Lampasona, Vito ;
Piemonti, Lorenzo ;
Ciceri, Fabio ;
Bilguvar, Kaya ;
Lifton, Richard P. ;
Vasse, Marc ;
Smadja, David M. ;
Migaud, Melanie ;
Hadjadj, Jerome ;
Terrier, Benjamin ;
Duffy, Darragh ;
Quintana-Murci, Lluis ;
van de Beek, Diederik ;
Roussel, Lucie ;
Vinh, Donald C. ;
Tangye, Stuart G. .
SCIENCE, 2020, 370 (6515) :423-+
[7]   Neutrophil Transmigration Mediated by the Neutrophil-Specific Antigen CD177 Is Influenced by the Endothelial S536N Dimorphism of Platelet Endothelial Cell Adhesion Molecule-1 [J].
Bayat, Behnaz ;
Werth, Silke ;
Sachs, Ulrich J. H. ;
Newman, Debra K. ;
Newman, Peter J. ;
Santoso, Sentot .
JOURNAL OF IMMUNOLOGY, 2010, 184 (07) :3889-3896
[8]   Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19 [J].
Blanco-Melo, Daniel ;
Nilsson-Payant, Benjamin E. ;
Liu, Wen-Chun ;
Uhl, Skyler ;
Hoagland, Daisy ;
Moller, Rasmus ;
Jordan, Tristan X. ;
Oishi, Kohei ;
Panis, Maryline ;
Sachs, David ;
Wang, Taia T. ;
Schwartz, Robert E. ;
Lim, Jean K. ;
Albrecht, Randy A. ;
tenOever, Benjamin R. .
CELL, 2020, 181 (05) :1036-+
[9]   Immune Alterations in a Patient with SARS-CoV-2-Related Acute Respiratory Distress Syndrome [J].
Bouadma, Lila ;
Wiedemann, Aurelie ;
Patrier, Juliette ;
Surenaud, Mathieu ;
Wicky, Paul-Henri ;
Foucat, Emile ;
Diehl, Jean-Luc ;
Hejblum, Boris P. ;
Sinnah, Fabrice ;
de Montmollin, Etienne ;
Lacabaratz, Christine ;
Thiebaut, Rodolphe ;
Timsit, J. F. ;
Levy, Yves .
JOURNAL OF CLINICAL IMMUNOLOGY, 2020, 40 (08) :1082-1092
[10]   Increased Plasma PCSK9 Levels Are Associated with Reduced Endotoxin Clearance and the Development of Acute Organ Failures during Sepsis [J].
Boyd, John H. ;
Fjell, Christopher D. ;
Russell, James A. ;
Sirounis, Demetrios ;
Cirstea, Mihai S. ;
Walley, Keith R. .
JOURNAL OF INNATE IMMUNITY, 2016, 8 (02) :211-220