Improvement of fermentation performance of Gluconobacter oxydans by combination of enhanced oxygen mass transfer in compressed-oxygen-supplied sealed system and cell-recycle technique

被引:58
作者
Zhou, Xin
Zhou, Xuelian
Xu, Yong [1 ]
机构
[1] Nanjing Forestry Univ, Coll Chem Engn, 159 Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Oxygen transfer rate (OTR); Compressed oxygen-supplied sealed stirred tank reactor (COS-SSTR); Cell-recycle; Gluconobacter oxydans; Xylonic acid; XYLONIC ACID; BIOTECHNOLOGICAL APPLICATIONS; DISSOLVED-OXYGEN; BIOREACTOR; STRATEGY; BROTH;
D O I
10.1016/j.biortech.2017.08.107
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Oxygen supply for microbial cultures is often identified as a limiting factor for aerobic fermentation. Through implementation of an integrated oxygen control strategy, the high oxygen mass transfer rate satisfied cellular metabolic demands. Gluconobacter oxydans NL71 fermentation of xylose to xylonic acid was improved remarkably. Finally, the productivity of xylonic acid from xylose by biooxidation was markedly increased to 32.5 +/- 3.1 g/L/h compared to production levels using conventional laboratory-scale bioreactors. By improving microbial fermentative vitality, we successfully bio-converted 1800 g xylose to 1813 +/- 36 g xylonic acid by combination of a fed-batch addition of xylose substrate as well as a cell-recycling strategy. Bioconversion results demonstrated a highly efficient fermentation model that performs continuous bioreaction, assisting the effort to industrialize microbial xylonic acid production.
引用
收藏
页码:1137 / 1141
页数:5
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