Preparation of a hydroxypropyl Ganoderma lucidum polysaccharide and its physicochemical properties

被引：78
作者：
Liu, Wei [1 ,2 ]
Xu, Jing [1 ]
Jing, Pu [3 ]
Yao, Wenbing [1 ]
Gao, Xiangdong [1 ]
Yu, Liangli [2 ]
机构：
[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China
[2] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA
[3] Jiangsu Univ, Dept Food Sci & Engn, Zhenjiang 212013, Peoples R China
来源：
FOOD CHEMISTRY | 2010年 / 122卷 / 04期
基金：
美国国家科学基金会; 中国国家自然科学基金;
关键词：
Hydroxypropyl; Modification; Ganoderma lucidum; Polysaccharide; Radical scavenging; ANTIOXIDANT ACTIVITIES; ANTITUMOR ACTIVITIES; MEDICINAL MUSHROOMS; IN-VITRO; CARBOXYMETHYLATION; STARCH; SCLEROTIUM; CHITOSAN; GLUCANS;
D O I：
10.1016/j.foodchem.2009.11.087
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A hydroxypropyl Ganoderma lucidum polysaccharide (H-GLP) was prepared from a low-value water-insoluble polysaccharide from G. lucidum (GLP) using propylene oxide under an alkaline condition. The H-GLP was characterised for its chemical structure with IR and C-13 NMR spectra and analysed for its mono-sugar composition, molecular weight, and hydroxypropyl content. H-GLP contained mannose, glucose, and galactose in a mole ratio of 1.0:36.5:3.59, respectively, with an average molecular weight of 788 kDa and a hydroxypropyl content of 12.05%. H-GLP also had an excellent water solubility of more than 50 mg/mL, suggesting that hydroxypropylation might serve as an effective approach to enhance water solubility of GLP. H-GLP was also compared to the original GLP and ascorbic acid for antioxidant properties. H-GLP showed much stronger free radical scavenging capacity against hydroxyl and superoxide anion radicals and hydrogen peroxide than GLP. These results suggested the potential of hydroxypropylation in developing water-soluble antioxidative polysaccharides from GLP to enhance the profitability of G. lucidium production and processing industries. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：965 / 971
页数：7
相关论文
共 29 条
[11]   Effect of hydroxypropylation on the properties of white yam (Dioscorea rotundata) starch [J].
Lawal, Olayide S. ;
Ogundiran, Olusegun O. ;
Adesogan, Ezekiel K. ;
Ogunsanwo, Babatunde M. ;
Sosanwo, Olumide A. .
STARCH-STARKE, 2008, 60 (07) :340-348
[12]   Antioxidant properties of several medicinal mushrooms [J].
Mau, JL ;
Lin, HC ;
Chen, CC .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (21) :6072-6077
[13]   Potential of a novel polysaccharide preparation (GLPP) from Anhui-grown Ganoderma lucidum in tumor treatment and immunostimulation [J].
Pang, X. ;
Chen, Z. ;
Gao, X. ;
Liu, W. ;
Slavin, M. ;
Yao, W. ;
Yu, L. L. .
JOURNAL OF FOOD SCIENCE, 2007, 72 (06) :S435-S442
[14]   Preparation and antimicrobial activity of hydroxypropyl chitosan [J].
Peng, YF ;
Han, BQ ;
Liu, WS ;
Xu, XJ .
CARBOHYDRATE RESEARCH, 2005, 340 (11) :1846-1851
[15]   Carboxymethylation of hemicelluloses isolated from sugarcane bagasse [J].
Ren, Jun-Li ;
Sun, Run-Cang ;
Peng, Feng .
POLYMER DEGRADATION AND STABILITY, 2008, 93 (04) :786-793
[16]   FLAVONOIDS ARE SCAVENGERS OF SUPEROXIDE ANIONS [J].
ROBAK, J ;
GRYGLEWSKI, RJ .
BIOCHEMICAL PHARMACOLOGY, 1988, 37 (05) :837-841
[17]   Carboxymethylation of cashew tree exudate polysaccharide [J].
Silva, DA ;
de Paula, RCM ;
Feitosa, JPA ;
de Brito, ACF ;
Maciel, JS ;
Paula, HCB .
CARBOHYDRATE POLYMERS, 2004, 58 (02) :163-171
[18]   HYDROXYL RADICAL SCAVENGING ACTIVITY OF COMPATIBLE SOLUTES [J].
SMIRNOFF, N ;
CUMBES, QJ .
PHYTOCHEMISTRY, 1989, 28 (04) :1057-1060
[19]   STRUCTURES AND ANTITUMOR ACTIVITIES OF THE POLYSACCHARIDES ISOLATED FROM FRUITING BODY AND THE GROWING CULTURE OF MYCELIUM OF GANODERMA-LUCIDUM [J].
SONE, Y ;
MISAKI, A .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1985, 49 (09) :2641-2653
[20]   Medicinal mushrooms and cancer therapy - translating a traditional practice into Western medicine [J].
Sullivan, R ;
Smith, JE ;
Rowan, NJ .
PERSPECTIVES IN BIOLOGY AND MEDICINE, 2006, 49 (02) :159-170
123