Transcriptome analysis of hemocytes from the white shrimp Litopenaeus vannamei with the injection of dopamine

被引:22
作者
Cun Wei [1 ]
Pan Luqing [1 ]
Xin Zhang [1 ]
Xu, Lijun [1 ]
Si, Lingjun [1 ]
Tong, Ruixue [1 ]
Wang, Hongdan [1 ]
机构
[1] Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Shandong, Peoples R China
关键词
Litopenaeus vannamei; Hemocyte; Dopamine; Neuroendocrine-immune system; Transcriptome; C-TYPE LECTIN; TIGER SHRIMP; PENAEUS-MONODON; IMMUNE-RESPONSE; GENE-EXPRESSION; DIFFERENTIAL EXPRESSION; PROPHENOLOXIDASE SYSTEM; ENHANCING PHAGOCYTOSIS; CARBOXYLESTERASE CDNAS; SIGNALING PATHWAYS;
D O I
10.1016/j.fsi.2019.09.043
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
As a crucial neuroendocrine-immune factor, dopamine (DA) could regulate the immune system of Litopenaeus vannamei. To understand the immune mechanisms and regulatory pathways of DA in L. vannamei, the transcriptome analysis of hemocytes of L. vannamei with injection of DA (10(-6) mol/shrimp) at 3 and 12 h were performed in this study. Moreover, quantitative real-time PCR (qPCR) method was applied to validate the accuracy of transcriptome sequencing and analyze the expression pattern of candidate differentially expressed genes (DEGs) at different time points (0, 3, 6, 12, and 24 h) after DA injection. The results showed that a total of 51382 unigenes with a N50 length of 2341 bp were generated. And 1397 and 457 DEGs were obtained by comparative transcriptome at 3 and 12h respectively. Moreover, the results of functional annotation and enriched pathway showed that the DEGs were involved in phagosome (ko04145), lysosome (ko04142), Endocytosis (ko04144), and NOD-like receptor signaling pathway (ko04621). Besides, the Pearson's correlation coefficient (R) between transcriptome sequencing and qPCR was 0.845, which confirmed the reliability of the transcriptome sequencing results and the accuracy of assembly. Furthermore, the expression pattern of 15 candidate DEGs, containing 9 up-regulated and 6 down-regulated DEGs at 3 h, indicated the regulation of DA in physiological functions especially in the immune system. Therefore, these results revealed that DA induced the expressions of membrane receptors or proteins, activated intracellular signaling pathways, regulated cellular and humoral immune systems, controlled antioxidation and apoptosis, and was involved in the regulation of neuroendocrine system. These findings are helpful to promote the understanding on the effects of biogenic amines on physiological functions and regulatory networks of crustacean, and offer a substantial material and foundation for researching the immune response of crustacean.
引用
收藏
页码:497 / 509
页数:13
相关论文
共 84 条
  • [1] A novel clip domain serine proteinase PmClipSP2 functions in shrimp prophenoloxidase system and acts as a scavenger protein for lipopolysaccharide
    Amparyup, P.
    Promrungreang, K.
    Charoensapsri, W.
    Sutthangkul, J.
    Tassanakajon, A.
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2013, 34 (06) : 1637 - 1637
  • [2] Scavenger receptor B protects shrimp from bacteria by enhancing phagocytosis and regulating expression of antimicrobial peptides
    Bi, Wen-Jie
    Li, Dian-Xiang
    Xu, Yi-Hui
    Xu, Sen
    Li, Jing
    Zhao, Xiao-Fan
    Wang, Jin-Xing
    [J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2015, 51 (01) : 10 - 21
  • [3] MAPK/p38 regulation of cytoskeleton rearrangement accelerates induction of macrophage activation by TLR4, but not TLR3
    Bian, Hongjun
    Li, Feifei
    Wang, Wenwen
    Zhao, Qi
    Gao, Shanshan
    Ma, Jincai
    Li, Xiao
    Ren, Wanhua
    Qin, Chengyong
    Qi, Jianni
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2017, 40 (05) : 1495 - 1503
  • [4] Booze RM, 2000, NEUROSCI RES COMMUN, V27, P45
  • [5] Fast and sensitive protein alignment using DIAMOND
    Buchfink, Benjamin
    Xie, Chao
    Huson, Daniel H.
    [J]. NATURE METHODS, 2015, 12 (01) : 59 - 60
  • [6] Cloning and characterization of the SpLRR cDNA from green mud crab, Scylla paramamosain
    Cao, Jingsong
    Lin, Zhongyang
    Zhang, Yueling
    Zhang, Xiaoyu
    Li, Shengkang
    Zhang, Nan
    Zou, Wenhui
    Li, Yuanyou
    Chen, Jiehui
    Wang, Xiuying
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2013, 34 (01) : 129 - 135
  • [7] Effects of dopamine on the immunity of white shrimp Litopenaeus vannamei
    Cheng, W
    Chieu, HT
    Tsai, CH
    Chen, JC
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2005, 19 (04) : 375 - 385
  • [8] Cathepsins and their involvement in immune responses
    Conus, Sebastien
    Simon, Hans-Uwe
    [J]. SWISS MEDICAL WEEKLY, 2010, 140 : 4 - 11
  • [9] A role of cathepsin L gene in innate immune response of crayfish (Procambarus clarkii)
    Dai, Li-Shang
    Chu, Sheng-Hui
    Yu, Xiao-Min
    Li, Yan-Yan
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2017, 71 : 246 - 254
  • [10] Transcriptome analysis of the molting gland (Y-organ) from the blackback land crab, Gecarcinus lateralis
    Das, Sunetra
    Pitts, Natalie L.
    Mudron, Megan R.
    Durica, David S.
    Mykles, Donald L.
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2016, 17 : 26 - 40