Neutral lipid alterations in Human Herpesvirus 8-infected HUVEC cells and their possible involvement in neo-angiogenesis

被引：24

作者：

Angius, Fabrizio ^{[1
]}

Uda, Sabrina ^{[1
]}

Piras, Enrica ^{[1
]}

Spolitu, Stefano ^{[1
]}

Ingianni, Angela ^{[1
]}

Batetta, Barbara ^{[1
]}

Pompei, Raffaello ^{[1
]}

机构：

[1] Univ Cagliari, Dept Biomed Sci, I-09124 Cagliari, Italy

来源：

BMC MICROBIOLOGY | 2015年 / 15卷

关键词：

Herpesvirus; 8; Endothelial cells; Viral infection; Cholesteryl esters; Triglycerides; Lipid droplets; Angiogenesis; SARCOMA-ASSOCIATED HERPESVIRUS; HEPATITIS-C VIRUS; KAPOSIS-SARCOMA; ENDOTHELIAL-CELLS; PROSTATE-CANCER; NILE-RED; INFECTION; HUMAN-HERPESVIRUS-8; EXPRESSION; DISEASES;

D O I：

10.1186/s12866-015-0415-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Human Herpesvirus 8 (HHV8), the causative agent of Kaposi's sarcoma, induces an intense modification of lipid metabolism and enhances the angiogenic process in endothelial cells. In the present study, neutral lipid (NL) metabolism and angiogenesis were investigated in HHV8-infected HUVEC cells. The viral replication phases were verified by rtPCR and also by K8.1 and LANA immunostaining. Results: Lipid droplets (Nile Red) were higher in all phases and NL staining (LipidTOX) combined with viral-antigen detection (immunofluorescence) demonstrated a NL content increase in infected cells. In particular, triglyceride synthesis increases in the lytic phase, whereas cholesteryl ester synthesis rises in the latent one. Moreover, the inhibition of cholesterol esterification reduces neo-tubule formation mainly in latently infected cells. Conclusions: We suggest that a reprogramming of cholesteryl ester metabolism is involved in regulating neo-angiogenesis in HHV8-infected cells and plays a likely role in the high metastatic potential of derived-tumours.

引用

页数：10

共 37 条

[1] Spectrum of Kaposi's sarcoma-associated herpesvirus, or human herpesvirus 8, diseases [J].