Differential control of cellular gene expression by diffusible and non-diffusible EGF

被引：60

作者：

Ito, Y ^{[1
]}

Chen, GP

Imanishi, Y

Morooka, T

Nishida, E

Okabayashi, Y

Kasuga, M

机构：

[1] Univ Tokushima, Fac Engn, Dept Biol Sci & Technol, JST,PRESTO, Tokushima 7708506, Japan

[2] Kyoto Univ, Grad Sch Engn, Dept Chem Mat, Kyoto 6066501, Japan

[3] NAIST, Grad Sch Mat Sci, Ikoma 6300101, Japan

[4] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 6068502, Japan

[5] Kobe Univ, Sch Med, Dept Internal Med 2, Kobe, Hyogo 6500017, Japan

来源：

JOURNAL OF BIOCHEMISTRY | 2001年 / 129卷 / 05期

关键词：

EGF; immobilized growth factor; juxtacrine; matricrine; PC12;

D O I：

10.1093/oxfordjournals.jbchem.a002913

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell gene expression is affected by both the kind and mode of growth factor stimulation (diffusive vs. non-diffusive). Epidermal growth factor (EGF) was pattern-immobilized on a polystyrene plate. Although the growth of the rat phaeochromocytoma cell line PC12 is stimulated by diffusible EGF, and differentiation is stimulated by diffusible nerve growth factor (NGF), immobilized (non-diffusible) EGF stimulated PC12 differentiation. The immobilized EGF caused a long-lasting stimulation of the intracellular signal protein mitogen-associated protein MAP kinase (MAPK, also known as ERK) and p38 (a subfamily of the MAPK superfamily) in cells, as did diffusible NGF. The switching between growth stimulation and differentiation is considered to be due to the duration of the stimulus. The function of the biosignal conjugate was regulated using conjugation methodology.

引用

页码：733 / 737

页数：5

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