Selective targeting of newly synthesized Arc mRNA to active synapses requires NMDA receptor activation

被引：359

作者：

Steward, O ^{[1
]}

Worley, PF

机构：

[1] Univ Calif Irvine, Coll Med, Reeve Irvine Res Ctr, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Coll Med, Dept Anat & Neurobiol, Irvine, CA 92697 USA

[3] Univ Calif Irvine, Coll Med, Dept Neurobiol & Behav, Irvine, CA 92697 USA

[4] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

来源：

NEURON | 2001年 / 30卷 / 01期

关键词：

D O I：

10.1016/S0896-6273(01)00275-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Newly synthesized Are mRNA is selectively targeted to synapses that have experienced particular patterns of activity. Here, we demonstrate that the targeting requires NMDA receptor activation. Are expression was induced by an electroconvulsive seizure, and the newly synthesized mRNA was then targeted to synaptic sites by activating the perforant path projections to the dentate gyrus. When micropipette electrodes containing NMDA receptor antagonists (MK801 or APV) were positioned in the dentate gyrus during the stimulation period, newly synthesized Are mRNA was transported into dendrites but did not localize in the activated lamina; instead, the mRNA remained diffusely distributed. AMPA receptor antagonists (CNQX) blocked targeting of Are mRNA in a small region, and mGluR antagonists (MCPG) did not affect localization. These results demonstrate that NMDA receptor activation is required for the targeting of Are mRNA to active synapses.

引用

页码：227 / 240

页数：14