FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis

被引：48

作者：

Hua, Rong ^{[1
]}

Wei, Huafang ^{[2
,3
]}

Liu, Chao ^{[2
,3
]}

Zhang, Yue ^{[1
]}

Liu, Siyu ^{[1
]}

Guo, Yueshuai ^{[1
]}

Cui, Yiqiang ^{[1
]}

Zhang, Xin ^{[1
]}

Guo, Xuejiang ^{[1
]}

Li, Wei ^{[2
,3
]}

Liu, Mingxi ^{[1
]}

机构：

[1] Nanjing Med Univ, Ctr Global Hlth, State Key Lab Reprod Med, Nanjing 211166, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

NUCLEIC ACIDS RESEARCH | 2019年 / 47卷 / 22期

基金：

中国国家自然科学基金;

关键词：

MEIOTIC CHROMOSOME DYNAMICS; COHESIN SMC1-BETA; BOUQUET FORMATION; MICE LACKING; MOUSE; PROTEIN; PROPHASE; RECOMBINATION; ATTACHMENT; COMPLEX;

D O I：

10.1093/nar/gkz992

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During meiosis, telomere attachment to the inner nuclear envelope is required for proper pairing of homologous chromosomes and recombination. Here, we identified F-box protein 47 (FBXO47) as a regulator of the telomeric shelterin complex that is specifically expressed during meiotic prophase I. Knockout of Fbxo47 in mice leads to infertility in males. We found that the Fbxo47 deficient spermatocytes are unable to form a complete synaptonemal complex. FBXO47 interacts with TRF1/2, and the disruption of Fbxo47 destabilizes TRF2, leading to unstable telomere attachment and slow traversing through the bouquet stage. Our findings uncover a novel mechanism of FBXO47 in telomeric shelterin subunit stabilization during meiosis.

引用

页码：11755 / 11770

页数：16

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