Interleukin-10 production by B cells is regulated by cytokines, but independently of GATA-3 or FoxP3 expression

被引：5

作者：

Bohacova, Pavla ^{[1
,2
]}

Kossl, Jan ^{[1
,2
]}

Hajkova, Michaela ^{[1
,2
]}

Hermankova, Barbora ^{[1
,2
]}

Javorkova, Eliska ^{[1
,2
]}

Zajicova, Alena ^{[2
]}

Krulova, Magdalena ^{[1
,2
]}

Holan, Vladimir ^{[1
,2
]}

机构：

[1] Czech Acad Sci, Dept Transplantat Immunol, Inst Expt Med, Videnska 1083, Prague 14220 4, Czech Republic

[2] Charles Univ Prague, Fac Sci, Dept Cell Biol, Prague, Czech Republic

来源：

CELLULAR IMMUNOLOGY | 2020年 / 347卷

关键词：

B cell; T cell; IL-10; production; GATA-3; FoxP3; Cytokine; T-CELLS; IFN-GAMMA; GENE-EXPRESSION; AUTOIMMUNITY; IL-10; MACROPHAGES; RESPONSES; BALANCE;

D O I：

10.1016/j.cellimm.2019.103987

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The knowledge of mechanisms of regulation of IL-10 production by B cells remains still very limited. We show here that highly purified mouse B cells stimulated with LPS produce significant levels of IL-10, but Bregs in our model do not express detectable level of either Foxp3 or GATA-3. Nevertheless, IL-10 production by B cells is regulated by cytokines. In activated B cells, IL-10 production was significantly enhanced by IFN-gamma and decreased in the presence of IL-4 or TGF-beta. These findings are in sharp contrast with the observations in T cells, where IL-10 production correlates with GATA-3 or FoxP3 expression, and the cytokines regulate IL-10 production in a reverse manner than in activated B cells. These results thus show that the production of IL-10 by Bregs is regulated by cytokines independently of the expression of GATA-3 and FoxP3, which is clearly different from GATA-3-dependent IL-10 production by activated Th2 cells and FoxP3 expression in IL-10-producing Tregs.

引用

页数：8

共 32 条

[1] Development of thymic Foxp3+ regulatory T cells: TGF-β matters [J].