Identification of expressed genes by laser-mediated manipulation of single cells

被引：341
|
作者：
Schütze, K [1 ]
Lahr, G [1 ]
机构：
[1] Acad Hosp Munchen Harlaching, Dept Med 1, Laser Lab & Mol Biol, Munich, Germany
来源：
NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 08期
关键词：
gene analysis technology; in situ analysis; RT-PCR;
D O I：
10.1038/nbt0898-737
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We describe a rapid noncontact method for the capture of single cells or small tissue areas of any size or shape directly within the cap of a common microfuge tube. Prior to the laser-mediated transfer, the specimen is isolated by laser microbeam microdissection, forming a clear-cut gap around the selected area. Laser treatment does not impair subsequent RNA analysis. We have used this method to isolate a single cell from archival colon adenocarcinoma, and were able to detect point mutations within codon 12 of c-Ki-ras2 mRNA after nested RT-PCR analysis.
引用
收藏
页码：737 / 742
页数：6
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