The expression profile of Chi-1, Glu-2, Glu-3 and PR1.2 genes in Scab-resistant and susceptible wheat cultivars during infection by Fusarium graminearum

Fusarium head blight (FHB) is one of the most destructive diseases of wheat (Triticum aestivum L.) in the world. Importance of this disease is due to yield reduction and contamination of grains to FHB mycotoxin harvested from infective ears which are dangerous to human and animals. The goal of the present study was evaluation of few famous PR proteins in wheat defense response to Fusarium graminearum in 'Falat' as a highly susceptible and 'Sumai3' as a resistant cultivar. The expression pattern of Chi-1, Glu-2, Glu-3 and PR1.2 genes have been studied during wheat defense reaction to Fusarium graminearum in Falat and Sumai3 as susceptible and resistant wheat cultivars, respectively. Infected spikes are sampled in 1, 3 and 7 days after artificial inoculation and subjected to total RNA extraction and cDNA synthesis. Quantitative Real-time PCR analysis showed Chi-1, Glu-2, Glu-3 genes was significantly down regulated in the susceptible cultivar, whereas their expression were significantly increased in the resistant cultivar. The transcript of PR1.2 gene exhibited a particular pattern and was induced significantly in 7 days after inoculation in the resistance cultivar. Result showed that respected encoded PR proteins might play important roles in defense reaction of wheat to Fusarium graminearum.