Two quenching groups are better than one: A robust strategy for constructing HOCl fluorescent probe with minimized background fluorescence and ultra-high sensitivity and its application of HOCl imaging in living cells and tissues

被引：25

作者：

Zheng, Guansheng ^{[1
,2
,3
]}

Li, Zejun ^{[1
]}

Duan, Qinya ^{[1
]}

Cheng, Ke ^{[2
,3
,4
]}

He, Yong ^{[1
]}

Huang, Shumei ^{[1
]}

Zhang, Huatang ^{[1
]}

Jiang, Yin ^{[1
]}

Jia, Yongguang ^{[5
]}

Sun, Hongyan ^{[2
,3
,4
]}

机构：

[1] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Sch Biomed & Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China

[2] City Univ Hong Kong, Dept Chem, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China

[3] City Univ Hong Kong, Ctr Super Diamond & Adv Films COSDAF, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China

[4] City Univ Hong Kong, Shenzhen Res Inst, Biotech & Hlth Ctr, Key Lab Biochip Technol, Shenzhen 518057, Peoples R China

[5] South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2020年 / 310卷

基金：

中国国家自然科学基金;

关键词：

Dual-quenching; Dual-reactive; HOCl; Ultra-sensitivity; Tissue imaging; HYPOCHLOROUS ACID DETECTION; EXCITATION CROSS-SECTIONS; DENSITY-FUNCTIONAL THEORY; TURN-ON; RAPID DETECTION; 2-PHOTON PROBE; FAST-RESPONSE; H2S SYNTHESIS; LIVE-CELL; MYELOPEROXIDASE;

D O I：

10.1016/j.snb.2020.127890

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report herein fluorescent probes equipped with dual-quenching groups exhibiting superior sensitivity than probes with mono-quenching groups. Importantly, with this strategy, the probe with dual-quenching groups react with HOCl through two distinct reaction mechanisms, which reduce the plausible side reactions with other competing analytes and enhance the probe's selectivity. As a proof-of-concept study, we designed and synthesized a probe with dual-quenching groups DQ-HOCl to detect HOCl, which is one of the most important ROS and linked with a number of diseases. In addition, two control probes with mono-quenching groups, MQ-HOCl-1 and MQ-HOCl-2, were also synthesized for comparison purpose. Fluorescent assays demonstrated that DQ-HOCl indeed shows ultra-high sensitivity and selectivity compared with probes with mono-quenching groups. Furthermore, the probe has been successfully utilized to imaging exogenous/endogenous HOCl in living cells. Moreover, DQ-HOCl was applied to visualize HOCl in kidney tissues from rat due to the increased penetration depth and lower tissue autofluorescence from the nature of two-photon probes.

引用

页数：11