Non-invasive intravital imaging of head and neck squamous cell carcinomas in live mice

被引：8

作者：

Amornphimoltham, Panomwat ^{[2
]}

Thompson, Jamie ^{[2
]}

Melis, Nicolas ^{[1
]}

Weigert, Roberto ^{[1
,2
]}

机构：

[1] NCI, Lab Cellular & Mol Biol, Ctr Canc Res, NIH, 37 Convent Dr Rm 2050B, Bethesda, MD 20892 USA

[2] Natl Inst Dent & Craniofacial Res, Intracellular Membrane Trafficking Sect, NIH, Bethesda, MD USA

来源：

METHODS | 2017年 / 128卷

关键词：

MICROSCOPY; METASTASIS;

D O I：

10.1016/j.ymeth.2017.07.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Head and neck squamous cell carcinoma is one of the most common cancers with a 50% 5-yearsurvival rate. Understanding the mechanisms that control development, progression, and spreading of the tumor to distal sites is of paramount importance to develop effective therapies. Here, we describe a minimally invasive procedure, which enables performing intravital microscopy of the mouse tongue in models for oral cancer and provides structural and dynamic information of the tumors at cellular and subcellular level. Published by Elsevier Inc.

引用

页码：3 / 11

页数：9

共 19 条

[1] A retroinhibition approach reveals a tumor cell-autonomous response to rapamycin in head and neck cancer [J].

Amornphimoltham, Panomwat ;

Patel, Vyomesh ;

Leelahavanichkul, Kantima ;

Abraham, Robert T. ;

Gutkind, J. Silvio .

CANCER RESEARCH, 2008, 68 (04) :1144-1153

[2] Rab25 Regulates Invasion and Metastasis in Head and Neck Cancer [J].

Amornphimoltham, Panomwat ;

Rechache, Kamil ;

Thompson, Jamie ;

Masedunskas, Andrius ;

Leelahavanichkul, Kantima ;

Patel, Vyomesh ;

Molinolo, Alfredo ;

Gutkind, J. Silvio ;

Weigert, Roberto .

CLINICAL CANCER RESEARCH, 2013, 19 (06) :1375-1388

[3] Intravital microscopy as a tool to study drug delivery in preclinical studies [J].

Amornphimoltham, Panomwat ;

Masedunskas, Andrius ;

Weigert, Roberto .

ADVANCED DRUG DELIVERY REVIEWS, 2011, 63 (1-2) :119-128

[4] Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues [J].

Berton, TR ;

Matsumoto, T ;

Page, A ;

Conti, CJ ;

Deng, CX ;

Jorcano, JL ;

Johnson, DG .

ONCOGENE, 2003, 22 (35) :5415-5426

[5] Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation [J].

Brown, E ;

McKee, T ;

diTomaso, E ;

Pluen, A ;

Seed, B ;

Boucher, Y ;

Jain, RK .

NATURE MEDICINE, 2003, 9 (06) :796-800

[6] Targeting Mammalian Target of Rapamycin by Rapamycin Prevents Tumor Progression in an Oral-Specific Chemical Carcinogenesis Model [J].

Czeminski, Rakefet ;

Amornphimoltham, Panomwat ;

Patel, Vyomesh ;

Molinolo, Alfredo A. ;

Gutkind, J. Silvio .

CANCER PREVENTION RESEARCH, 2009, 2 (01) :27-36

[7]

Gligorijevic B., 2009, J Vis Exp

[8] Fluorescence lifetime imaging and reflectance confocal microscopy for multiscale imaging of oral precancer [J].

Jabbour, Joey M. ;

Cheng, Shuna ;

Malik, Bilal H. ;

Cuenca, Rodrigo ;

Jo, Javier A. ;

Wright, John ;

Cheng, Yi-Shing Lisa ;

Maitland, Kristen C. .

JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (04)

[9] Intravital two-photon microscopy for studying the uptake and trafficking of fluorescently conjugated molecules in live rodents [J].

Masedunskas, Andrius ;

Weigert, Roberto .

TRAFFIC, 2008, 9 (10) :1801-1810

[10] A global double-fluorescent cre reporter mouse [J].

Muzumdar, Mandar Deepak ;

Tasic, Bosiljka ;

Miyamichi, Kazunari ;

Li, Ling ;

Luo, Liqun .

GENESIS, 2007, 45 (09) :593-605

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