A Quantitative 3D Motility Analysis of Trypanosoma brucei by Use of Digital In-line Holographic Microscopy

被引：28

作者：

Weisse, Sebastian ^{[1
,2
]}

Heddergott, Niko ^{[3
]}

Heydt, Matthias ^{[1
]}

Pflaesterer, Daniel ^{[1
]}

Maier, Timo ^{[1
,4
]}

Haraszti, Tamas ^{[1
,4
]}

Grunze, Michael ^{[1
,2
]}

Engstler, Markus ^{[3
]}

Rosenhahn, Axel ^{[1
,2
]}

机构：

[1] Univ Heidelberg, Heidelberg, Germany

[2] Karlsruhe Inst Technol, Inst Funct Interfaces, IFG, Karlsruhe, Germany

[3] Univ Wurzburg, Dept Cell & Dev Biol, Bioctr, Wurzburg, Germany

[4] Max Planck Inst Intelligent Syst, Stuttgart, Germany

来源：

PLOS ONE | 2012年 / 7卷 / 05期

关键词：

AFRICAN TRYPANOSOMES; ACTIN CORTEX; FLAGELLUM; TRACKING; SURFACE; MODELS;

D O I：

10.1371/journal.pone.0037296

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We present a quantitative 3D analysis of the motility of the blood parasite Trypanosoma brucei. Digital in-line holographic microscopy has been used to track single cells with high temporal and spatial accuracy to obtain quantitative data on their behavior. Comparing bloodstream form and insect form trypanosomes as well as mutant and wildtype cells under varying external conditions we were able to derive a general two-state-run-and-tumble-model for trypanosome motility. Differences in the motility of distinct strains indicate that adaption of the trypanosomes to their natural environments involves a change in their mode of swimming.

引用

页数：13

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