Development of the Sterile Insect Technique to control the dengue vector Aedes aegypti (Linnaeus) in Sri Lanka

被引:14
作者
Ranathunge, Tharaka [1 ,2 ]
Harishchandra, Jeevanie [3 ]
Maiga, Hamidou [4 ]
Bouyer, Jeremy [4 ]
Gunawardena, Y. I. Nilmini Silva [1 ]
Hapugoda, Menaka [1 ]
机构
[1] Univ Kelaniya, Mol Med Unit, Fac Med, Colombo, Sri Lanka
[2] CINEC Campus, Dept Biomed Sci, Fac Hlth Sci, Malabe, Sri Lanka
[3] Minist Hlth, Antimalaria Campaign AMC Publ Hlth Complex, Colombo, Sri Lanka
[4] IAEA, Dept Nucl Sci & Applicat, Insect Pest Control Subprogramme, Joint FAO IAEA Ctr Nucl Tech Food & Agr, Vienna, Austria
来源
PLOS ONE | 2022年 / 17卷 / 04期
关键词
ANOPHELES-STEPHENSI; GAMMA-RADIATION; DIPTERA;
D O I
10.1371/journal.pone.0265244
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background The Sterile Insect Technique (SIT) is presently being tested to control dengue in several countries. SIT aims to cause the decline of the target insect population through the release of a sufficient number of sterilized male insects. This induces sterility in the female population, as females that mate with sterilized males produce no offspring. Male insects are sterilized through the use of ionizing irradiation. This study aimed to evaluate variable parameters that may affect irradiation in mosquito pupae. Methods An Ae. aegypti colony was maintained under standard laboratory conditions. Male and female Ae. aegypti pupae were separated using a Fay and Morlan glass sorter and exposed to different doses of gamma radiation (40, 50, 60, 70 and 80 Gy) using a Co-60 source. The effects of radiation on survival, flight ability and the reproductive capacity of Ae. aegypti were evaluated under laboratory conditions. In addition, mating competitiveness was evaluated for irradiated male Ae. aegypti mosquitoes to be used for future SIT programmes in Sri Lanka. Results Survival of irradiated pupae was reduced by irradiation in a dose-dependent manner but it was invariably greater than 90% in control, 40, 50, 60, 70 Gy in both male and female Ae. aegypti. Irradiation didn't show any significant adverse effects on flight ability of male and female mosquitoes, which consistently exceeded 90%. A similar number of eggs per female was observed between the non-irradiated groups and the irradiated groups for both irradiated males and females. Egg hatch rates were significantly lower when an irradiation dose above 50 Gy was used as compared to 40 Gy in both males and females. Irradiation at higher doses significantly reduced male and female survival when compared to the non-irradiated Ae. aegypti mosquitoes. Competitiveness index (C) scores of sterile and non-sterile males compared with non-irradiated male mosquitoes under laboratory and semi-field conditions were 0.56 and 0.51 respectively at 50 Gy. Signification Based on the results obtained from the current study, a 50 Gy dose was selected as the optimal radiation dose for the production of sterile Ae. aegypti males for future SIT-based dengue control programmes aiming at the suppression of Ae. aegypti populations in Sri Lanka.
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页数:15
相关论文
共 55 条
[1]  
Adliene D., 2017, Applications of Ionizing Radiation in Materials Processing, V1, P55
[2]  
AKRAM M, 1975, Pakistan Journal of Zoology, V7, P177
[3]   The unprecedented magnitude of the 2017 dengue outbreak in Sri Lanka provides lessons for future mosquito-borne infection control and prevention [J].
Ali, Shahid ;
Khan, Abdul Waheed ;
Taylor-Robinson, Andrew W. ;
Adnan, Muhammad ;
Malik, Shahana ;
Gul, Saba .
INFECTION DISEASE & HEALTH, 2018, 23 (02) :114-120
[4]  
[Anonymous], 2013, INT J CURR MICROBIOL
[5]   Control of the olive fruit fly using genetics-enhanced sterile insect technique [J].
Ant, Thomas ;
Koukidou, Martha ;
Rempoulakis, Polychronis ;
Gong, Hong-Fei ;
Economopoulos, Aris ;
Vontas, John ;
Alphey, Luke .
BMC BIOLOGY, 2012, 10
[6]  
Arredondo J., 2016, Proceedings of the 9th International Symposium on Fruit Flies of Economic Importance, 12-16 May 2014, Bangkok, Thailand, P355
[7]  
Bakri A, 2005, STERILE INSECT TECHNIQUE: PRINCIPLES AND PRACTICE IN AREA-WIDE INTEGRATED PEST MANAGEMENT, P233, DOI 10.1007/1-4020-4051-2_9
[8]  
Bakri A., 2008, AREA WIDE STERILE IN, V12, P2015
[9]   Mosquitoborne infections after Hurricane Jeanne, Haiti, 2004 [J].
Beatty, Mark E. ;
Hunsperger, Elizabeth ;
Long, Earl ;
Schurch, Julia ;
Jain, Seema ;
Colindres, Rom ;
Lerebours, Gerald ;
Bernard, Yves-Marie ;
Dobbins, James Goodman ;
Brown, Mathew ;
Clark, Gary G. .
EMERGING INFECTIOUS DISEASES, 2007, 13 (02) :308-310
[10]  
Bolker B, GLMM FAQ TESTING OVE