Prevalence and genetic diversity of Babesia and Anaplasma species in cattle in Sudan

被引：35
作者：
Awad, Hoyam [2 ]
Antunes, Sandra [1 ]
Galindo, Ruth C. [3 ]
do Rosario, Virgilio E. [1 ]
de la Fuente, Jose [3 ,4 ]
Domingos, Ana [1 ]
El Hussein, Abdelrahim M. [2 ]
机构：
[1] Inst Higiene & Med Trop, P-1349008 Lisbon, Portugal
[2] Cent Vet Res Labs, Khartoum, Sudan
[3] Inst Invest Recursos Cineget IREC CSIC UCLM JCCM, Ciudad Real 13071, Spain
[4] Oklahoma State Univ, Coll Vet Med, Dept Vet Pathobiol, Stillwater, OK 74078 USA
来源：
VETERINARY PARASITOLOGY | 2011年 / 181卷 / 2-4期
关键词：
Babesia bigemina; Babesia bovis; Anaplasma marginale; Molecular diagnosis; Ticks; PCR-BASED DETECTION; BOVINE BABESIOSIS; BIGEMINA; MARGINALE; THEILERIA; PARASITES; PIROPLASMS; ANIMALS; STRAINS; REGION;
D O I：
10.1016/j.vetpar.2011.04.007
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Disease prevalence studies are one of the most valuable tools to demonstrate the risk or impact of certain infections in local and global economies. The data obtained in these studies contribute to develop strategies for disease control. The present study aims to provide information about the prevalence of babesiosis and anaplasmosis in the northern regions of Sudan. Blood samples from four different states of Sudan were collected from apparently healthy cattle (n = 692), DNA was extracted and the prevalence of Babesia and Anaplasma species was analyzed by PCR. The results confirmed the presence of Babesia bigemina, Babesia bovis and Anaplasma marginale in cattle in northern Sudan with overall prevalence rates of 4.0%, 1.9% and 6.1%, respectively. Statistical analysis revealed that the prevalence of B. bigemina, B. bovis and A. marginale varies significantly between Sudanese states as well as in different age groups, while gender seems not to have a significant effect on the prevalence of these pathogens among Sudanese cattle. The highest prevalence for B. bigemina was found in the Aljazirah State while the highest number of A. marginale positive samples was reported in River Nile. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：146 / 152
页数：7
相关论文
共 30 条
[11]   Molecular phylogeny and biogeography of North American isolates of Anaplasma marginale (Rickettsiaceae: Ehrlichieae) [J].
de la Fuente, J ;
Van den Bussche, RA ;
Kocan, KM .
VETERINARY PARASITOLOGY, 2001, 97 (01) :65-76
[12]  
de la Fuente J, 2007, VET MICROBIOL, V119, P375, DOI [10.1016/j.vetmic.2006.09.011, 10.1016/j.vetmic.2006.09.015]
[13]  
de la Fuente Jose, 2005, Anim Health Res Rev, V6, P75, DOI 10.1079/AHR2005104
[14]   Expressed sequence tag (EST) analysis of the erythrocytic stages of Babesia bovis [J].
de Vries, Erik ;
Corton, Craig ;
Harris, Barbara ;
Cornelissen, Albert W. C. A. ;
Berriman, Matthew .
VETERINARY PARASITOLOGY, 2006, 138 (1-2) :61-74
[15]   MULTIPLEX POLYMERASE CHAIN-REACTION BASED ASSAY FOR THE DETECTION OF BABESIA-BIGEMINA, BABESIA-BOVIS AND ANAPLASMA-MARGINALE DNA IN BOVINE BLOOD [J].
FIGUEROA, JV ;
CHIEVES, LP ;
JOHNSON, GS ;
BUENING, GM .
VETERINARY PARASITOLOGY, 1993, 50 (1-2) :69-81
[16]  
Gayo V, 2003, ONDERSTEPOORT J VET, V70, P197
[17]   Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization [J].
Gubbels, JM ;
De Vos, AP ;
Van der Weide, M ;
Viseras, J ;
Schouls, LM ;
De Vries, E ;
Jongejan, F .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) :1782-1789
[18]   Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites [J].
Iseki, Hiroshi ;
Alhassan, Andy ;
Ohta, Naomi ;
Thekisoe, Oriel M. M. ;
Yokoyama, Naoaki ;
Inoue, Noboru ;
Nambota, Andrew ;
Yasuda, Jun ;
Igarashi, Ikuo .
JOURNAL OF MICROBIOLOGICAL METHODS, 2007, 71 (03) :281-287
[19]   Antigens and alternatives for control of Anaplasma marginale infection in cattle [J].
Kocan, KM ;
de la Fuente, J ;
Guglielmone, AA ;
Meléndez, RD .
CLINICAL MICROBIOLOGY REVIEWS, 2003, 16 (04) :698-+
[20]   Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method [J].
Martins, Tiago M. ;
Pedro, Olivia C. ;
Caldeira, Rubina A. ;
do Rosario, Virgilio E. ;
Neves, Luis ;
Domingos, Ana .
VETERINARY PARASITOLOGY, 2008, 153 (3-4) :225-230
123