Chiral resolution of two potential aromatase inhibitors on cellulose tris (4-methyl benzoate) and cellulose tris (3,5-dimethylphenyl carbamate) chiral stationary phases

The chiral resolution of 1-[(benzofuran-2-yl)-5-nitrophenyl methyl] tetrazole (1) and 1-[(benzofuran-2-yl)- 5-bromophenylmethyl] tetrazole (II), the novel aromatase inhibitors, has been achieved on Chiracel OD, Chiracel OD-RH columns which are coated with cellulose [tris (3,5-dimethylphenyl carbamate)] and Chiracel OJ-R column coated with cellulose [tris (4-methyl benzoate)]. The mobile phases used were hexane-2-propanol (95:5) on Chiracel OD and water-acetonitrile (45:55) on Chiracel OD-RH and Chiracel OJ-R columns respectively. Compound I has; been resolved on Chiracel OD (alpha = 1.30 and Rs = 3.13) and OD-RH (alpha = 1.15 and Rs = 1.20) columns while compound II has been resolved on OJ-R (alpha = 1.31 and Rs = 1.80) column only. The effect of acetonitrile concentration in water-acetonitrile mobile phase was studied on the retention and resolution of both compounds.