Evaluation of the Sulfo-Succinimidyl-4-(N-Maleimidomethyl) Cyclohexane-1-Carboxylate Coupling Chemistry for Attachment of Oligonucleotides to Magnetic Nanobeads

被引：8
作者：
de la Torre, Teresa Zardan Gomez [1 ]
Herthnek, David [2 ]
Ramachandraiah, Harisha [2 ]
Svedlindhl, Peter [1 ]
Nilsson, Mats [2 ]
Stromme, Maria [1 ]
机构：
[1] Uppsala Univ, Dept Engn Sci, Angstrom Lab, SE-75121 Uppsala, Sweden
[2] Uppsala Univ, Dept Genet & Pathol, Rudbeck Lab, SE-75185 Uppsala, Sweden
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2011年 / 11卷 / 10期
基金：
瑞典研究理事会;
关键词：
DNA Detection; Oligonucleotide Coupling Chemistry; Sulfo-SMCC; Magnetic Nanobeads; Biosensor; DNA; NANOPARTICLES; REPLICATION;
D O I：
10.1166/jnn.2011.5667
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The sulfo-SMCC (Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate) coupling chemistry was evaluated for immobilization of oligonucleotides onto 130 nm sized magnetic nanobeads aimed for bio-detection in a magnetic readout assay. The chemistry was found to produce a high surface coverage of similar to 93 +/- 10 oligonucleotides per bead whereas stability tests showed that about 50% of the oligonucleotides detached from the bead surfaces after eight weeks of storage in a buffer solution. It was shown that bead aggregation prior to magnetic readout could be suppressed by incubating the samples at 70 degrees C for 30 min. The same temperature was also shown to be the most favorable for hybridization between the oligonucleotide functionalized beads and rolling circle amplified DNA molecules. This should simplify the heating procedure in a biosensor in which hybridization and magnetic readout is performed in the same compartment.
引用
收藏
页码：8532 / 8537
页数：6
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