Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction

被引：11

作者：

Estrozi, L. F. ^{[2
]}

Neumann, E. ^{[2
]}

Squires, G. ^{[3
]}

Rozas-Dennis, G. ^{[5
]}

Costabel, M. ^{[6
]}

Rey, F. A. ^{[4
]}

Guerin, D. M. A. ^{[1
]}

Navaza, J. ^{[2
]}

机构：

[1] Univ Basque Country, EHU CSIC, Unidad Biofis, E-48080 Bilbao, Spain

[2] Univ Grenoble 1, CNRS, CEA, Inst Biol Struct Jean Pierre Ebel,IST, F-38027 Grenoble, France

[3] Inst Curie, F-75248 Paris 05, France

[4] Inst Pasteur, F-75724 Paris, France

[5] Univ Nacl Sur, Dept Biol Bioquim & Farm, RA-8000 Bahia Blanca, Buenos Aires, Argentina

[6] Univ Nacl Sur, Dept Fis, Grp Biofis, RA-8000 Bahia Blanca, Buenos Aires, Argentina

来源：

VIROLOGY | 2008年 / 375卷 / 01期

关键词：

cryo-EM; 3D reconstruction; Triatoma virus; combining X-ray; EM data;

D O I：

10.1016/j.virol.2007.12.025

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The blood-sucking reduviid bug Triatoma infestans, one of the most important vector of American human trypanosomiasis (Chagas disease) is infected by the Triatoma virus (TrV). TrV has been classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work presents the three-dimensional cryo-electron microscopy (cryo-EM) reconstruction of the TrV capsid at about 25 A resolution and its use as a template for phasing the available crystallographic data by the molecular replacement method. The main structural differences between the cryo-EM reconstruction of TrV and other two viruses, one from the same family, the cricket paralysis virus (CrPV) and the human rhinovirus 16 from the Picornaviridae family are presented and discussed. (c) 2008 Published by Elsevier Inc.

引用

页码：85 / 93

页数：9

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