Development of a multiplex real-time polymerase chain reaction for the detection of influenza virus type A including H5 and H9 subtypes

被引：22

作者：

Li, Pei-qiong ^{[1
,2
]}

Zhang, Jun ^{[1
,2
]}

Muller, Claude P. ^{[3
]}

Chen, Jing-xian ^{[4
]}

Yang, Zi-feng ^{[4
]}

Zhang, Ren ^{[5
]}

Li, Juan ^{[1
,2
]}

He, Yun-shao ^{[1
]}

机构：

[1] Sun Yat Sen Univ, DaAn Gene Diagnost Ctr, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Dept Anat, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China

[3] Natl Publ Hlth Lab, Inst Immunol, L-1011 Luxembourg, Luxembourg

[4] Guangzhou Inst Resp Dis, Virus Lab, Guangzhou 510120, Guangdong, Peoples R China

[5] Guangzhou Univ Chinese Med, Guangzhou 510407, Guangdong, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 02期

关键词：

avian influenza virus; diagnose method; multiplex real-time polymerase chain reaction;

D O I：

10.1016/j.diagmicrobio.2008.01.007

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Avian influenza viruses (AIVs) are endemic in wild birds and, if transmitted to poultry, can cause serious economic losses. The aim of this study was to develop a multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) for rapid detection of influenza virus type A. including H5 and H9 subtypes. The selected primers and various labeled TaqMan reporter probes corresponding to matrix, H5, and H9 genes were used in a multiplex real-time RT-PCR to simultaneously detect triple fluorescent signals. The results showed that the multiplex real-time RT-PCR assay can be applied to detect RNA of influenza virus type A including H5 and H9 subtypes with a high specificity and a sensitivity of 10 copies per reaction. As a result of its short turnaround times and a high specificity and sensitivity, the assay is very suitable for large-scale screening during AIV outbreaks. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：192 / 197

页数：6

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