Specific detection of Lawsonia intracellularis in porcine proliferative enteropathy inferred from fluorescent rRNA in situ hybridization

被引：35

作者：

Boye, M ^{[1
]}

Jensen, TK ^{[1
]}

Moller, K ^{[1
]}

Leser, TD ^{[1
]}

Jorsal, SE ^{[1
]}

机构：

[1] Danish Vet Lab, DK-1790 Copenhagen V, Denmark

来源：

VETERINARY PATHOLOGY | 1998年 / 35卷 / 02期

关键词：

indirect immunofluorescence assay; in situ hybridization; Lawsonia intracellularis; porcine proliferative enteropathy;

D O I：

10.1177/030098589803500212

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.

引用

页码：153 / 156

页数：4

共 8 条

[1] COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].