Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics

被引：50

作者：

Liu, Wei ^{[1
,2
]}

Xu, Jinnan ^{[1
,2
]}

Zhu, Rui ^{[1
,2
]}

Zhu, Yiqing ^{[1
,2
]}

Zhao, Yang ^{[3
]}

Chen, Pei ^{[3
]}

Pan, Chun ^{[4
]}

Yao, Wenbing ^{[1
,2
]}

Gao, Xiangdong ^{[1
,2
]}

机构：

[1] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Peoples R China

[2] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China

[3] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA

[4] Southeast Univ, Zhongda Hosp, Dept Crit Care Med, Nanjing 210009, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 78卷

基金：

中国国家自然科学基金;

关键词：

Lycium barbarum; Fingerprinting profile; Chemometrics; GYNOSTEMMA-PENTAPHYLLUM; QUANTIFICATION; FRUIT; HPLC; EXPRESSION; ZEAXANTHIN;

D O I：

10.1016/j.ijbiomac.2015.03.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Techniques including ultraviolet-visible spectra (UV), high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were used in the fingerprinting analysis of Lycium barbarum polysaccharides (LBPs) from different locations and varieties. Multiple fingerprinting profiles were used to evaluate the similarity and classification of different LBPs with the help of chemometrics. The results indicated that sixteen batches of LBPs had good consistency, and fingerprinting techniques were simple and robust for quality control of LBPs as well as related products. In addition, fingerprinting techniques combined with chemometrics could also be used to identify different cultivation locations of LBPs samples. Finally, four monosaccharides (galacturonic acid, glucose, galactose and arabinose) and the absorptions of stretching vibration of ester carbonyl groups as well as N-H variable angle vibration of-CONH-could be selected as herbal markers to distinguish different samples. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：230 / 237

页数：8

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