A novel splicing isoform of mouse sterol regulatory element-binding protein-1 (SREBP-1)

被引：3

作者：

Inoue, J ^{[1
]}

Sato, R ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Biochem & Mol Biol Lab, Suita, Osaka 5650871, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 01期

关键词：

SREBP; transcription factor; splicing;

D O I：

10.1271/bbb.63.243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned a cDNA encoding the NH2-terminal portion of mouse SREBP-1. The deduced amino acid sequence was 76% and 90% identical to human and hamster SREBP-1, respectively. We found out a novel splicing isoform of mouse SREBP-1 that lacks 42 amino acid residues composing a PEST sequence observed in unstable proteins. It has been reported that SREBP-1 is rapidly turned over in the nucleus. Although this isoform was not a dominant isoform, it might be possible that the produced protein functions differently from other isoforms including a complete PEST sequence.

引用

页码：243 / 245

页数：3

共 17 条

[1] STEROL REGULATION OF FATTY-ACID SYNTHASE PROMOTER - COORDINATE FEEDBACK-REGULATION OF 2 MAJOR LIPID PATHWAYS [J].

BENNETT, MK ;

LOPEZ, JM ;

SANCHEZ, HB ;

OSBORNE, TF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (43) :25578-25583

[2] MOLECULAR-CLONING AND FUNCTIONAL-ANALYSIS OF THE PROMOTER OF THE HUMAN SQUALENE SYNTHASE GENE [J].

GUAN, GM ;

JIANG, GJ ;

KOCH, RL ;

SHECHTER, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (37) :21958-21965

[3] STRUCTURE OF THE HUMAN GENE ENCODING STEROL REGULATORY ELEMENT-BINDING PROTEIN-1 (SREBF1) AND LOCALIZATION OF SREBF1 AND SREBF2 TO CHROMOSOMES 17P11.2 AND 22Q13 [J].

HUA, XX ;

WU, J ;

GOLDSTEIN, JL ;

BROWN, MS ;

HOBBS, HH .

GENOMICS, 1995, 25 (03) :667-673

[4] SREBP-2, A 2ND BASIC-HELIX-LOOP-HELIX-LEUCINE ZIPPER PROTEIN THAT STIMULATES TRANSCRIPTION BY BINDING TO A STEROL REGULATORY ELEMENT [J].

HUA, XX ;

YOKOYAMA, C ;

WU, J ;

BRIGGS, MR ;

BROWN, MS ;

GOLDSTEIN, JL ;

WANG, XD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) :11603-11607

[5] NF-Y HAS A NOVEL ROLE IN STEROL-DEPENDENT TRANSCRIPTION OF 2 CHOLESTEROGENIC GENES [J].

JACKSON, SM ;

ERICSSON, J ;

OSBORNE, TF ;

EDWARDS, PA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (37) :21445-21448

[6] Sterol regulation of acetyl coenzyme A carboxylase: A mechanism for coordinate control of cellular lipid [J].

Lopez, JM ;

Bennett, MK ;

Sanchez, HB ;

Rosenfeld, JM ;

Osborne, TF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (03) :1049-1053

[7] Epidermal growth factor and okadaic acid stimulate Sp1 proteolysis [J].

Mortensen, ER ;

Marks, PA ;

Shiotani, A ;

Merchant, JL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (26) :16540-16547

[8] A CATALOG OF SPLICE JUNCTION SEQUENCES [J].

MOUNT, SM .

NUCLEIC ACIDS RESEARCH, 1982, 10 (02) :459-472

[9]

OSBORNE TF, 1988, J BIOL CHEM, V263, P3380

[10] Sterol-dependent transcriptional regulation of sterol regulatory element-binding protein-2 [J].

Sato, R ;

Inoue, J ;

Kawabe, Y ;

Kodama, T ;

Takano, T ;

Maeda, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (43) :26461-26464

← 1 2 →