Molecular and functional characterization of goldfish (Carassius auratus L.) transforming growth factor beta

被引：78
作者：
Haddad, George [1 ]
Hanington, Patrick C. [1 ]
Wilson, Elaine C. [1 ]
Grayfer, Leon [1 ]
Belosevic, Miodrag [1 ,2 ]
机构：
[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
[2] Univ Alberta, Dept Publ Hlth Sci, Edmonton, AB T6G 2E9, Canada
来源：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2008年 / 32卷 / 06期
基金：
加拿大自然科学与工程研究理事会;
关键词：
transforming growth; factor-beta; TGF-beta; fish; goldfish; macrophages; de-activation; proliferation; inflammation; innate immunity;
D O I：
10.1016/j.dci.2007.10.003
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Transforming growth factor beta (TGF-beta) is a pleiotropic cytokine with important roles in the regulation of cell proliferation, differentiation, survival, migration, activation and deactivation. It is one of the first cytokines released during an immune response and plays a strong immunomodulatory role in the activation and subsequent de-activation of macrophages and other immune cells. TGF-beta is a highly conserved molecule, and members of the TGF superfamily can be found in organisms as evolutionarily distant as arthropods. In this manuscript, we described the identification of a goldfish TGF-beta molecule, which was highly expressed in the skin, kidney and spleen of the goldfish and its expression was up-regulated in macrophages treated with LPS or recombinant goldfish TNF-alpha. Goldfish TGF-beta shared a high amino acid identity with, and was phylogenetically related to, TGF-beta 1 of other teleost fish, birds, amphibians and mammals. Recombinant goldfish TGF-beta (rTGF-beta) induced the protiferation of a goldfish fibroblast cell line (CCL71) in a dose-dependent manner. In addition, rTGF-beta down-regulated the nitric oxide response of TNIF-alpha-activated macrophages. This is the first report of teleost TGF-beta function in an ectothermic vertebrate. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：654 / 663
页数：10
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