Cold case: The disappearance of Egypt bee virus, a fourth distinct master strain of deformed wing virus linked to honeybee mortality in 1970's Egypt

被引：26

作者：

de Miranda, Joachim R. ^{[1
]}

Brettell, Laura E. ^{[2
,3
,24
]}

Chejanovsky, Nor ^{[4
]}

Childers, Anna K. ^{[5
]}

Dalmon, Anne ^{[6
]}

Deboutte, Ward ^{[7
,8
]}

de Graaf, Dirk C. ^{[9
]}

Doublet, Vincent ^{[10
,11
]}

Gebremedhn, Haftom ^{[9
,12
]}

Genersch, Elke ^{[13
,14
]}

Gisder, Sebastian ^{[14
]}

Granberg, Fredrik ^{[15
]}

Haddad, Nizar J. ^{[16
]}

Kaden, Rene ^{[1
,17
]}

Manley, Robyn ^{[10
]}

Matthijnssens, Jelle ^{[7
]}

Meeus, Ivan ^{[18
]}

Migdadi, Hussein ^{[16
]}

Milbrath, Meghan O. ^{[1
]}

Mondet, Fanny ^{[6
]}

Remnant, Emily J. ^{[19
]}

Roberts, John M. K. ^{[20
]}

Ryabov, Eugene, V ^{[5
]}

Sela, Noa ^{[4
]}

Smagghe, Guy ^{[18
]}

Somanathan, Hema ^{[21
]}

Wilfert, Lena ^{[10
,11
]}

Wright, Owen N. ^{[22
]}

Martin, Stephen J. ^{[3
,23
]}

Ball, Brenda, V ^{[23
]}

机构：

[1] Swedish Univ Agr Sci, Dept Ecol, S-75007 Uppsala, Sweden

[2] Western Sydney Univ, Hawkesbury Inst Environm, Locked Bag 1797, Renrith, NSW 2751, Australia

[3] Univ Salford, Sch Environm & Life Sci, Manchester M5 4WT, Lancs, England

[4] Volcani Ctr, Inst Plant Protect, POB 15159, IL-7528809 Rishon Leziyyon, Israel

[5] USDA, Bee Res Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA

[6] INRAE, Abeilles & Environm, F-84914 Avignon, France

[7] Univ Leuven, Rega Inst, Dept Microbiol Immunol & Transplantat, Lab Clin & Epidemiol Virol, B-3000 Leuven, Belgium

[8] Wax Planck Inst Immunobiol & Epigenet, Stubeweg 51, D-79108 Freiburg, Germany

[9] Univ Ghent, Lab Mol Entomol & Bee Pathol, B-9000 Ghent, Belgium

[10] Univ Exeter, Coll Life & Environm Sci, Penryn TR10 9FE, England

[11] Univ Ulm, Inst Evolutionary Ecol & Conservat Genom, Albert Einstein Allee 11, D-89081 Ulm, Germany

[12] Tigray Agr Res Inst, POB 492, Mekelle, Ethiopia

[13] Free Univ Berlin, Fachbereich Vet Med, Inst Mikrobiol & Terseuchen, Berlin, Germany

[14] Inst Bee Res, Dept Mol Microbiol & Bee Dis, Hohen Neuendorf, Germany

[15] Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, S-75007 Uppsala, Sweden

[16] Natl Agr Res Ctr, Bee Res Dept, Baqa, Jordan

[17] Uppsala Univ, Dept Med Sci, Clin Microbiol, S-75309 Uppsala, Sweden

[18] Univ Ghent, Dept Plants & Crops, Lab Agrozool, Coupure Links 653, B-9000 Ghent, Belgium

[19] Univ Sydney, Sch Life & Environm Sci, Behav Ecol & Evolut BEE Lab, Camperdown, NSW 2006, Australia

[20] Commonwealth Sci & Ind Res Org, Canberra, ACT 2601, Australia

[21] Indian Inst Sci Educ & Res, Sch Biol, Thiruvananthapuram 695551, Kerala, India

[22] Univ Exeter, Coll Life & Environm Sci, Ctr Res Anim Behav, Exeter EX4 4QG, Devon, England

[23] Rothamsted Res, Harpenden AL5 2JQ, Herts, England

[24] Univ Liverpool Liverpool Sch Trop Med, Dept Vector Biol, Liverpool L3 5QA, Merseyside, England

来源：

VIROLOGY JOURNAL | 2022年 / 19卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 美国食品与农业研究所;

关键词：

Egypt bee virus; Deformed wing virus; Master strain; Varroa destructor; Honeybee; Apis mellifera; Western blot; RNA sequencing; Bioinformatic screening; PICORNA-LIKE VIRUS; VARROA-DESTRUCTOR; APIS-MELLIFERA; READ ALIGNMENT; SEQUENCE; RECOMBINANTS; PARASITE; RNA;

D O I：

10.1186/s12985-022-01740-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In 1977, a sample of diseased adult honeybees (Apis mellifera) from Egypt was found to contain large amounts of a previously unknown virus, Egypt bee virus, which was subsequently shown to be serologically related to deformed wing virus (DWV). By sequencing the original isolate, we demonstrate that Egypt bee virus is in fact a fourth unique, major variant of DWV (DWV-D): more closely related to DWV-C than to either DWV-A or DWV-B. DWV-A and DWV-B are the most common DWV variants worldwide due to their close relationship and transmission by Varroa destructor. However, we could not find any trace of DWV-D in several hundred RNA sequencing libraries from a worldwide selection of honeybee, varroa and bumblebee samples. This means that DWV-D has either become extinct, been replaced by other DWV variants better adapted to varroa-mediated transmission, or persists only in a narrow geographic or host range, isolated from common bee and beekeeping trade routes.

引用

页数：11

共 74 条

[31] Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data [J].

Kearse, Matthew ;

Moir, Richard ;

Wilson, Amy ;

Stones-Havas, Steven ;

Cheung, Matthew ;

Sturrock, Shane ;

Buxton, Simon ;

Cooper, Alex ;

Markowitz, Sidney ;

Duran, Chris ;

Thierer, Tobias ;

Ashton, Bruce ;

Meintjes, Peter ;

Drummond, Alexei .

BIOINFORMATICS, 2012, 28 (12) :1647-1649

[32] MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms [J].

Kumar, Sudhir ;

Stecher, Glen ;

Li, Michael ;

Knyaz, Christina ;

Tamura, Koichiro .

MOLECULAR BIOLOGY AND EVOLUTION, 2018, 35 (06) :1547-1549

[33]

Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/NMETH.1923, 10.1038/nmeth.1923]

[34] Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.) [J].

Lanzi, Gaetana ;

De Miranda, Joachim R. ;

Boniotti, Maria Beatrice ;

Cameron, Craig E. ;

Lavazza, Antonio ;

Capucci, Lorenzo ;

Camazine, Scott M. ;

Rossi, Cesare .

JOURNAL OF VIROLOGY, 2006, 80 (10) :4998-5009

[35] New Viruses from the Ectoparasite Mite Varroa destructor Infesting Apis mellifera and Apis cerana [J].

Levin, Sofia ;

Sela, Noa ;

Erez, Tal ;

Nestel, David ;

Pettis, Jeffery ;

Neumann, Peter ;

Chejanovsky, Nor .

VIRUSES-BASEL, 2019, 11 (02)

[36]

Li H, 2009, BIOINFORMATICS, V25, P1754, DOI [10.1093/bioinformatics/btp324, 10.1093/bioinformatics/btp698, 10.1093/bioinformatics/btp352]

[37] Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination [J].

Lole, KS ;

Bollinger, RC ;

Paranjape, RS ;

Gadkari, D ;

Kulkarni, SS ;

Novak, NG ;

Ingersoll, R ;

Sheppard, HW ;

Ray, SC .

JOURNAL OF VIROLOGY, 1999, 73 (01) :152-160

[38] Pathogen shifts in a honeybee predator following the arrival of the Varroa mite [J].

Loope, Kevin J. ;

Baty, James W. ;

Lester, Philip J. ;

Rankin, Erin E. Wilson .

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2019, 286 (1894)

[39] The EMBL-EBI search and sequence analysis tools APIs in 2019 [J].

Madeira, Fabio ;

Park, Young Mi ;

Lee, Joon ;

Buso, Nicola ;

Gur, Tamer ;

Madhusoodanan, Nandana ;

Basutkar, Prasad ;

Tivey, Adrian R. N. ;

Potter, Simon C. ;

Finn, Robert D. ;

Lopez, Rodrigo .

NUCLEIC ACIDS RESEARCH, 2019, 47 (W1) :W636-W641

[40] Knock-on community impacts of a novel vector: spillover of emerging DWV-B from Varroa-infested honeybees to wild bumblebees [J].

Manley, Robyn ;

Temperton, Ben ;

Doyle, Toby ;

Gates, Daisy ;

Hedges, Sophie ;

Boots, Michael ;

Wilfert, Lena .

ECOLOGY LETTERS, 2019, 22 (08) :1306-1315

← 1 2 3 4 5 6 7 8 →