Synthesis, cellular uptake, apopotosis, cytotoxicity, cell cycle arrest, interaction with DNA and antioxidant activity of ruthenium(II) complexes

被引：59
作者：
Huang, Hong-Liang [2 ]
Li, Zheng-Zheng [1 ]
Liang, Zhen-Hua [1 ]
Yao, Jun-Hua [3 ]
Liu, Yun-Jun [1 ,4 ]
机构：
[1] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Guangdong, Peoples R China
[2] Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Instrumentat Anal & Res Ctr, Guangzhou 510275, Guangdong, Peoples R China
[4] Guangdong Pharmaceut Univ, Sch Chem & Chem Engn, Guangzhou 510006, Guangdong, Peoples R China
来源：
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY | 2011年 / 46卷 / 08期
关键词：
Ruthenium(II) complexes; Cytotoxicity; Apoptosis; Cellular uptake; DNA-binding; DEOXYRIBONUCLEIC-ACID; POLYPYRIDYL COMPLEXES; BINDING; LIGAND; 1,10-PHENANTHROLINE; 2,2-BIPYRIDINE; PHOTOCLEAVAGE; APOPTOSIS; GROWTH; DRUGS;
D O I：
10.1016/j.ejmech.2011.04.049
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A new ligand and two ruthenium(II) complexes [Ru(bpy)(2)(DNPIP)](ClO4)(2) 1 and [Ru(bpy)(2)(DAPIP)](ClO4)(2) 2 were synthesized and characterized. The DNA-binding constants for complexes 1 and 2 were determined to be 2.24 (+/-0.30) x 10(5) M-1 (s = 1.29) and 6.34 (+/-0.32) x 10(4) M-1 (s = 2.84). The photocleavage of pBR322 DNA by Ru(II) complexes was investigated. The cytotoxicity of complexes 1 and 2 were assessed against three tumor cell lines. The apoptosis and cellular uptake were studied. The retardation assay of pGL 3 plasmid DNA was explored. The cell cycle arrest was analysized by flow cytometry. The antioxidant activities of the ligand and complexes were also investigated. Crown Copyright (C) 2011 Published by Elsevier Masson SAS. All rights reserved.
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收藏
页码：3282 / 3290
页数：9
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