Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

被引：127
作者：
Tamatani, T
Kobayashi, H
Tezuka, K
Sakamoto, S
Suzuki, K
Nakanishi, T
Takigawa, M
Miyano, T
机构：
[1] JT Inc, Pharmaceut Frontier Res Labs, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[2] Juntendo Univ, Sch Med, Dept Pediat Surg, Bunkyo Ku, Tokyo 113, Japan
[3] Okayama Univ, Sch Dent, Dept Biochem & Mol Dent, Okayama 7008525, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 251卷 / 03期
关键词：
D O I：
10.1006/bbrc.1998.9543
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-beta but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders. (C) 1998 Academic Press.
引用
收藏
页码：748 / 752
页数：5
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