Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

被引：127

作者：

Tamatani, T

Kobayashi, H

Tezuka, K

Sakamoto, S

Suzuki, K

Nakanishi, T

Takigawa, M

Miyano, T

机构：

[1] JT Inc, Pharmaceut Frontier Res Labs, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan

[2] Juntendo Univ, Sch Med, Dept Pediat Surg, Bunkyo Ku, Tokyo 113, Japan

[3] Okayama Univ, Sch Dent, Dept Biochem & Mol Dent, Okayama 7008525, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 251卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.9543

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-beta but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders. (C) 1998 Academic Press.

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页码：748 / 752

页数：5

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