Detection of PCR products in solution using surface plasmon resonance

被引：78

作者：

Kai, E

Sawata, S

Ikebukuro, K

Iida, T

Honda, T

Karube, I

机构：

[1] Univ Tokyo, Adv Sci & Technol Res Ctr, Meguro Ku, Tokyo 1538904, Japan

[2] Natl Space Dev Agcy Japan, Tsukuba, Ibaraki 3050047, Japan

[3] Osaka Univ, Microbial Dis Res Inst, Dept Bacterial Infect, Suita, Osaka 5650871, Japan

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 04期

关键词：

D O I：

10.1021/ac9807161

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Polymerase chain reaction (PCR) products were detected using a now injection-type sensor based on surface plasmon resonance. Asymmetric PCR was used to amplify the target DNA sequence, and two products with different length were produced. The novelty of our DNA detection system was that our target DNA was double stranded but the probe binding site, located in the 3'-terminus, was single stranded. This avoids the formation of intra- and intermolecular complexes. This novel design permitted us not only to detect PCR product but also to develop a rapid detection system for the detection of the verotoxin 2 gene of Escherichia coli O157:H7.

引用

页码：796 / 800

页数：5

共 32 条

[1] Fiber-optic evanescent wave biosensor for the detection of oligonucleotides [J].