Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

被引：42

作者：

Chen, Guangyu E. ^{[1
]}

Canniffe, Daniel P. ^{[1
,2
]}

Barnett, Samuel F. H. ^{[1
]}

Hollingshead, Sarah ^{[1
,3
]}

Brindley, Amanda A. ^{[1
]}

Vasilev, Cvetelin ^{[1
]}

Bryant, Donald A. ^{[2
]}

Hunter, C. Neil ^{[1
]}

机构：

[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Western Bank, Sheffield S10 2TN, S Yorkshire, England

[2] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[3] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX4 2DY, England

来源：

SCIENCE ADVANCES | 2018年 / 4卷 / 01期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会; 欧洲研究理事会;

关键词：

ISOCYCLIC RING FORMATION; RHODOBACTER-CAPSULATUS; BACTERIOCHLOROPHYLL BIOSYNTHESIS; RUBRIVIVAX-GELATINOSUS; HEME-BIOSYNTHESIS; PCC; 6803; PROTEIN; PLANTS; IDENTIFICATION; CYANOBACTERIA;

D O I：

10.1126/sciadv.aaq1407

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg-protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in Escherichia coli. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning E. coli cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.

引用

页数：8

共 41 条

[1] Attachment of Noncognate Chromophores to CpcA of Synechocystis sp PCC 6803 and Synechococcus sp PCC 7002 by Heterologous Expression in Escherichia coli [J].

Alvey, Richard M. ;

Biswas, Avijit ;

Schluchter, Wendy M. ;

Bryant, Donald A. .

BIOCHEMISTRY, 2011, 50 (22) :4890-4902

[2]

BEALE SI, 1994, CIBA F SYMP, V180, P156

[3] Regulation and function of tetrapyrrole biosynthesis in plants and algae [J].

Brzezowski, Pawel ;

Richter, Andreas S. ;

Grimm, Bernhard .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2015, 1847 (09) :968-985

[4] Three classes of oxygen-dependent cyclase involved in chlorophyll and bacteriochlorophyll biosynthesis [J].

Chen, Guangyu E. ;

Canniffe, Daniel P. ;

Hunter, C. Neil .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (24) :6280-6285

[5] Absence of the cbb3 Terminal Oxidase Reveals an Active Oxygen-Dependent Cyclase Involved in Bacteriochlorophyll Biosynthesis in Rhodobacter sphaeroides [J].

Chen, Guangyu E. ;

Canniffe, Daniel P. ;

Martin, Elizabeth C. ;

Hunter, C. Neil .

JOURNAL OF BACTERIOLOGY, 2016, 198 (15) :2056-2063

[6] A Cyanobacterial Chlorophyll Synthase-HliD Complex Associates with the Ycf39 Protein and the YidC/Alb3 Insertase [J].

Chidgey, Jack W. ;

Linhartova, Markta ;

Komenda, Josef ;

Jackson, Philip J. ;

Dickman, Mark J. ;

Canniffe, Daniel P. ;

Konik, Peter ;

Pilny, Jan ;

Hunter, C. Neil ;

Sobotka, Roman .

PLANT CELL, 2014, 26 (03) :1267-1279

[7] Assembly of the Photosystem II Membrane-Protein Complex of Oxygenic Photosynthesis [J].

Eaton-Rye, Julian J. ;

Sobotka, Roman .

FRONTIERS IN PLANT SCIENCE, 2017, 8

[8]

Fujita Y., 2017, Modern Topics in the Phototrophic Prokaryotes: Metabolism, Bioenergetics, and Omics, P67

[9] Anaerobic chlorophyll isocyclic ring formation in Rhodobacter capsulatus requires a cobalamin cofactor [J].

Gough, SP ;

Petersen, BO ;

Duus, JO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6908-6913

[10] Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination [J].

Gustafsson, Mats G. L. ;

Shao, Lin ;

Carlton, Peter M. ;

Wang, C. J. Rachel ;

Golubovskaya, Inna N. ;

Cande, W. Zacheus ;

Agard, David A. ;

Sedat, John W. .

BIOPHYSICAL JOURNAL, 2008, 94 (12) :4957-4970

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