Complete genome sequencing and genomic characterization of two Escherichia coli strains co-producing MCR-1 and NDM-1 from bloodstream infection

被引:23
|
作者
Zheng, Beiwen [1 ]
Yu, Xiao [1 ]
Xu, Hao [1 ]
Guo, Lihua [1 ]
Zhang, Jing [1 ,2 ]
Huang, Chen [1 ]
Shen, Ping [1 ]
Jiang, Xiawei [3 ]
Xiao, Yonghong [1 ]
Li, Lanjuan [1 ]
机构
[1] Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Diag & Treatment Infect Dis, Affiliated Hosp 1,Coll Med, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Resp Dis, Hangzhou, Zhejiang, Peoples R China
[3] Zhejiang Chinese Med Univ, Coll Basic Med Sci, Hangzhou, Zhejiang, Peoples R China
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
基金
中国国家自然科学基金;
关键词
RESISTANCE GENE MCR-1; PLASMID; COEXISTENCE; EMERGENCE; MEAT;
D O I
10.1038/s41598-017-18273-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We previously described the discovery of two Escherichia coli isolates (EC1002 and EC2474) co-harbouring mcr-1 and bla(NDM-1) genes, which were recovered from bloodstream infection in China. More importantly, these antibiotic resistance genes were located on different plasmids and signaling the potential spread of pandrug-resistant bacteria. Here, the complete genome sequences of both isolates were determined using Pacbio RS II and Illumina HiSeq2000 systems. The genome of EC1002 consists of a 5,177,501 base pair chromosome and four circular plasmids, while the genome of EC2474 consists of a 5,013,813 base pair chromosome and three plasmids. The plasmid replicon type of pEC1002_NDM and pEC2474_NDM were identified as IncA/C2 and IncF, respectively. The genetic environment of bla(NDM-1) in this study was similar to bla(NDM)-carrying plasmids detected in China, although the overall nucleotide identity and query coverage were variable. The plasmid replicon type of pEC1002_MCR and pEC2474_MCR were identified as IncI2 and IncHI2, respectively. Two different genetic strategies for mcr-1 gene spread were observed in this study and bla(NDM-1) genes were also found transferred by two different mobile genetic elements in two plasmids. The findings of this study further support that the diversified transfer mechanisms of bla(NDM-1) and mcr-1 present in Enterobacteriaceae.
引用
收藏
页数:7
相关论文
共 50 条
  • [1] Complete genome sequencing and genomic characterization of two Escherichia coli strains co-producing MCR-1 and NDM-1 from bloodstream infection
    Beiwen Zheng
    Xiao Yu
    Hao Xu
    Lihua Guo
    Jing Zhang
    Chen Huang
    Ping Shen
    Xiawei Jiang
    Yonghong Xiao
    Lanjuan Li
    Scientific Reports, 7
  • [2] Complete genome sequence and genomic characterization of two clinical Escherichia coli strains co-producing MCR-1 and NDM-1
    Yu, X.
    Zheng, B.
    Xu, H.
    Xiao, Y.
    INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2017, 50 : S102 - S102
  • [3] Characteristics of Carbapenem-Resistant and Colistin-Resistant Escherichia coli Co-Producing NDM-1 and MCR-1 from Pig Farms in China
    Peng, Zhong
    Li, Xiaosong
    Hu, Zizhe
    Li, Zugang
    Lv, Yujin
    Lei, Minggang
    Wu, Bin
    Chen, Huanchun
    Wang, Xiangru
    MICROORGANISMS, 2019, 7 (11)
  • [4] Coexistence of MCR-1 and NDM-1 in Clinical Escherichia coli Isolates
    Zheng, Beiwen
    Dong, Huihui
    Xu, Hao
    Lv, Jifang
    Zhang, Jing
    Jiang, Xiawei
    Du, Yu
    Xiao, Yonghong
    Li, Lanjuan
    CLINICAL INFECTIOUS DISEASES, 2016, 63 (10) : 1393 - U145
  • [5] Increased activity of colistin in combination with amikacin against Escherichia coli co-producing NDM-5 and MCR-1
    Zhou, Yu-Feng
    Tao, Meng-Ting
    Feng, Youjun
    Yang, Run-Shi
    Liao, Xiao-Ping
    Liu, Ya-Hong
    Sun, Jian
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2017, 72 (06) : 1723 - 1730
  • [6] Carbapenem-resistant and colistin-resistant Escherichia coli co-producing NDM-9 and MCR-1
    Yao, Xu
    Doi, Yohei
    Zeng, Li
    Lv, Luchao
    Liu, Jian-Hua
    LANCET INFECTIOUS DISEASES, 2016, 16 (03): : 288 - 289
  • [7] First report of Escherichia coli co-producing NDM-1 and OXA-232
    Both, Anna
    Huang, Jiabin
    Kaase, Martin
    Hezel, Julia
    Wertheimer, Daniel
    Fenner, Ines
    Guenther, Thomas
    Grundhoff, Adam
    Buettner, Henning
    Aepfelbacher, Martin
    Rohde, Holger
    Hentschke, Moritz
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2016, 86 (04) : 437 - 438
  • [8] Complete genome sequencing revealed novel genetic contexts of the mcr-1 gene in Escherichia coli strains
    Yu, Choo Yee
    Ang, Geik Yong
    Chong, Teik-Min
    San Chin, Pui
    Ngeow, Yun Fong
    Yin, Wai-Fong
    Chan, Kok-Gan
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2017, 72 (04) : 1253 - 1255
  • [9] Emergence of tigecycline resistance in Escherichia coli co-producing MCR-1 and NDM-5 during tigecycline salvage treatment
    Wang, Qian
    Zhang, Ping
    Zhao, Dongdong
    Jiang, Yan
    Zhao, Feng
    Wang, Yanfei
    Li, Xi
    Du, Xiaoxing
    Yu, Yunsong
    INFECTION AND DRUG RESISTANCE, 2018, 11 : 2241 - 2248
  • [10] Coproduction of MCR-1 and NDM-1 by Colistin-Resistant Escherichia coli Isolated from a Healthy Individual
    Zhong, Lan-Lan
    Zhang, Yan-Fen
    Doi, Yohei
    Huang, Xi
    Zhang, Xue-Fei
    Zeng, Kun-Jiao
    Shen, Cong
    Patil, Sandip
    Xing, Yong
    Zou, Yutian
    Tian, Guo-Bao
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2017, 61 (01)